catalog number :
MBS766123
products type :
ELISA Kit
products full name :
Human HIV (1+2 antibodies) ELISA Kit
products short name :
[HIV (1+2 antibodies)]
products gene name :
[HIV]
specificity :
This assay has high sensitivity and excellent specificity for detection of GHAb. No significant cross-reactivity or interference between GHAb and analogues was observed.
storage stability :
4 degree C for 6 months
other info1 :
Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids. Assay Type: Sandwich. Detection Range: 0.313-20ng/ml. Sensitivity: < 0.188ng/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level GHAb were tested 20 times on one plate, respectively. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level GHAb were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
products description :
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Antigen was pre-coated onto 96-well plates. And the Biotin-labeled Antigen was used as detection antigen. The standards, test samples and Biotin-labeled antigen were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin Conjugate was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the GHAb amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of GHAb can be calculated.
size4 :
10x96-Strip-Wells