ELISA/assay

Human HS (Heparan Sulfate)

MBS766118

48-Strip-Wells

300 USD

ELISA Kit

Human HS (Heparan Sulfate)

[HS (Heparan Sulfate)]

[HS]

Human

This assay has high sensitivity and excellent specificity for detection of Holo TC. No significant cross-reactivity or interference between Holo TC and analogues is observed.

4 degree C for 6 months

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids. Assay Type: Competitive. Detection Range: 0.156-10ng/ml. Sensitivity: <0.094ng/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Holo TC were tested 20 times on one plate, respectively. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Holo TC were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%

Principle of the Assay: This kit was based on Competitive-ELISA detection method. The microtiter plate provided in this kit has been pre-coated with Holo TC. During the reaction, Holo TC in the sample or standard competes with a fixed amount of Holo TC on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to Holo TC. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Holo TC in the samples is then determined by comparing the O D of the samples to the standard curve.

48-Strip-Wells

300 USD

96-Strip-Wells

415

5x96-Strip-Wells

1825

10x96-Strip-Wells

3425