catalog number :
MBS766110
products type :
ELISA Kit
products full name :
Human Anti HCV (Hepatitis C Virus Antibody)
products short name :
[Anti HCV (Hepatitis C Virus Antibody)]
products gene name :
[HCV]
specificity :
This assay has high sensitivity and excellent specificity for detection of Galactose-deficient IgA1. No significant cross-reactivity or interference between Galactose-deficient IgA1 and analogues was observed.
storage stability :
4 degree C for 6 months
other info1 :
Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids. Detection Range: 14.06-900ng/ml. Sensitivity: < 8ng/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Galactose-deficient IgA1 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Galactose-deficient IgA1 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
products description :
Principle of the Assay: This kit was based on indirect enzyme-linked immune-sorbent assay technology. Protein A was pre-coated onto 96-well plates. And the biotin conjugated anti-Human galactose-deficient IgA1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human galactose-deficient IgA1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human galactose-deficient IgA1 can be calculated.
size4 :
10x96-Strip-Wells