catalog number :
MBS766109
products type :
ELISA Kit
products full name :
Human TRACP-5b (Tartrate-resistant Acid Phosphatase 5b)
products short name :
[TRACP-5b (Tartrate-resistant Acid Phosphatase 5b)]
products name syn :
[MGC117378; TRAP; TrATPase tartrate resistant acid phosphatase 5 tartrate-resistant acid ATPase]
products gene name :
[TRACP-5b]
specificity :
This assay has high sensitivity and excellent specificity for detection of TRACP-5b. No significant cross-reactivity or interference between TRACP-5b and analogues was observed.
storage stability :
4 degree C for 6 months
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids. Assay Type: Sandwich. Detection Range: 0.781-50ng/ml. Sensitivity: <0.469ng/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level TRACP-5b were tested 20 times on one plate, respectively. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level TRACP-5b were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
products description :
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-TRACP-5b antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-TRACP-5b antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the TRACP-5b amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of TRACP-5b can be calculated.
size4 :
10x96-Strip-Wells