catalog number :
MBS766108
products type :
ELISA Kit
products full name :
Human MT (Melatonin)
products short name :
[MT (Melatonin)]
products gene name :
[MT]
specificity :
This assay has high sensitivity and excellent specificity for detection of MT. No significant crossreactivity or interference between MT and analogues was observed.
storage stability :
4 degree C for 6 months
image1 heading :
Typical Standard Curve
other info1 :
Samples: Serum, plasma and other biological fluids. Assay Type: Competitive ELISA, Coated with Antigen. Detection Range: 7.813-500pg/ml. Sensitivity: 4.688pg/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level MT were tested 20 times on one plate, respectively. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level MT were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
products description :
Principle of the Assay: This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with MT. During the reaction, MT in the sample or standard competes with a fixed amount of MT on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to MT. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin(SABC) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of MT in the samples is then determined by comparing the O.D. of the samples to the standard curve.
size4 :
10x96-Strip-Wells