catalog number :
MBS766086
products type :
ELISA Kit
products full name :
Human BDG (beta-D-glucan)
products short name :
[BDG (beta-D-glucan)]
products gene name :
[BDG]
specificity :
This assay has high sensitivity and excellent specificity for detection of BCL2. No significant cross-reactivity or interference between BCL2 and analogues was observed.
storage stability :
4 degree C for 6 months
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids. Assay Type: Sandwich. Detection Range: 78-5000pg/ml. Sensitivity: <46.9pg/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level BCL2 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level BCL2 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
products description :
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-BCL2 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-BCL2 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the BCL2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of BCL2 can be calculated.
size4 :
10x96-Strip-Wells