ELISA/assay

Human Glutamine synthetase ELISA Kit

MBS765948

48-Strip-Wells

300 USD

ELISA Kit

Human Glutamine synthetase ELISA Kit

[Glutamine synthetase]

[GLUL/GS/GLNS/Glutamine synthetase/Glutamate--ammonia ligase]

[glutamine synthetase; Glutamine synthetase; glutamine synthetase; glutamate-ammonia ligase; Glutamate decarboxylase (EC:4.1.1.15); Glutamate--ammonia ligase]

[GLUL]

[GLUL; GLUL; GS; GLNS; PIG43; PIG59; GLNS; GS]

GLNA_HUMAN

Human

373

This assay has high sensitivity and excellent specificity for detection of GLP1. No significant cross-reactivity or interference between GLP1 and analogues was observed.

Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids. Assay Type: Sandwich. Detection Range: 0.313-20ng/ml. Sensitivity: <0.188ng/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level GLP1 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level GLP1 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-GLP1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-GLP1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the GLP1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of GLP1 can be calculated.

74271837

NP_001028216.1

NM_001033044.3

42,064 Da

Alanine, Aspartate And Glutamate Metabolism Pathway (101142); Alanine, Aspartate And Glutamate Metabolism Pathway (100063); Amino Acid Synthesis And Interconversion (transamination) Pathway (1270159); Arginine Biosynthesis Pathway (82943); Arginine Biosynthesis Pathway (306); Astrocytic Glutamate-Glutamine Uptake And Metabolism Pathway (1268785); Biosynthesis Of Amino Acids Pathway (790012); Biosynthesis Of Amino Acids Pathway (795174); GABA Shunt Pathway (142350); GABAergic Synapse Pathway (377263)

The protein encoded by this gene belongs to the glutamine synthetase family. It catalyzes the synthesis of glutamine from glutamate and ammonia in an ATP-dependent reaction. This protein plays a role in ammonia and glutamate detoxification, acid-base homeostasis, cell signaling, and cell proliferation. Glutamine is an abundant amino acid, and is important to the biosynthesis of several amino acids, pyrimidines, and purines. Mutations in this gene are associated with congenital glutamine deficiency, and overexpression of this gene was observed in some primary liver cancer samples. There are six pseudogenes of this gene found on chromosomes 2, 5, 9, 11, and 12. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]

GLUL: This enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner. Essential for proliferation of fetal skin fibroblasts. Defects in GLUL are the cause of congenital systemic glutamine deficiency (CSGD). CSGD is a rare developmental disorder with severe brain malformation resulting in multi-organ failure and neonatal death. Glutamine is largely absent from affected patients serum, urine and cerebrospinal fluid. Belongs to the glutamine synthetase family. Protein type: Energy Metabolism - nitrogen; EC 4.1.1.15; Amino Acid Metabolism - arginine and proline; Ligase; Amino Acid Metabolism - alanine, aspartate and glutamate; EC 6.3.1.2. Chromosomal Location of Human Ortholog: 1q31. Cellular Component: cytoplasm; cytosol; mitochondrion; myelin sheath; nerve terminal; nucleus; perikaryon; protein complex; rough endoplasmic reticulum. Molecular Function: ATP binding; dynein light chain binding; glutamate binding; glutamate decarboxylase activity; glutamate-ammonia ligase activity; identical protein binding; magnesium ion binding; manganese ion binding. Biological Process: amino acid biosynthetic process; cell proliferation; cellular response to starvation; glutamate catabolic process; glutamine biosynthetic process; neurotransmitter uptake; positive regulation of epithelial cell proliferation; positive regulation of insulin secretion; positive regulation of synaptic transmission, glutamatergic; protein homooligomerization; response to glucose stimulus; synaptic transmission. Disease: Glutamine Deficiency, Congenital

48-Strip-Wells

300 USD

96-Strip-Wells

415

5x96-Strip-Wells

1825

10x96-Strip-Wells

3425