ELISA/assay

Human Neurofilament light polypeptide ELISA Kit

MBS765857

48-Strip-Wells

300 USD

ELISA Kit

Human Neurofilament light polypeptide ELISA Kit

[Neurofilament light polypeptide]

[NEFL/Neurofilament light polypeptide/NF-L/68 kDa neurofilament protein/Neurofilament triplet L protein]

[neurofilament light polypeptide; Neurofilament light polypeptide; neurofilament light polypeptide; neurofilament, light polypeptide; 68 kDa neurofilament protein; Neurofilament triplet L protein]

[NEFL]

[NEFL; NEFL; NFL; NF-L; NF68; CMT1F; CMT2E; PPP1R110; NF68; NFL; NF-L]

NFL_HUMAN

Human

543

This assay has high sensitivity and excellent specificity for detection of NEFH. No significant cross-reactivity or interference between NEFH and analogues was observed.

Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids. Assay Type: Sandwich. Detection Range: 3.12-200pg/ml. Sensitivity: <1.875pg/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level NEFH were tested 20 times on one plate, respectively. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level NEFH were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-NEFH antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-NEFH antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the NEFH amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of NEFH can be calculated.

105990539

NP_006149.2

NM_006158.4

61,517 Da

ARMS-mediated Activation Pathway (1269471); Activation Of NMDA Receptor Upon Glutamate Binding And Postsynaptic Events Pathway (1268798); Amyotrophic Lateral Sclerosis (ALS) Pathway (920975); Amyotrophic Lateral Sclerosis (ALS) Pathway (83099); Amyotrophic Lateral Sclerosis (ALS) Pathway (511); Axon Guidance Pathway (1270303); CREB Phosphorylation Through The Activation Of CaMKII Pathway (1268805); CREB Phosphorylation Through The Activation Of Ras Pathway (1268801); Cytokine Signaling In Immune System Pathway (1269310); DAP12 Interactions Pathway (1269283)

Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y. [provided by RefSeq, Oct 2008]

NFL: one of the three (L, M, and H) intermediate filament proteins that form neurofilaments. Neurofilaments are involved in the maintenance of neuronal caliber. NF-L is the most abundant of the three neurofilament proteins. Defects cause Charcot-Marie-Tooth disease type 1F (CMT1F). Protein type: Cytoskeletal. Chromosomal Location of Human Ortholog: 8p21. Cellular Component: axon; cytoplasm; cytosol; growth cone; myelin sheath; neurofilament; TSC1-TSC2 complex. Molecular Function: identical protein binding; phospholipase binding; protein binding; protein binding, bridging; protein C-terminus binding; protein domain specific binding; protein heterodimerization activity; structural constituent of cytoskeleton. Biological Process: activation of MAPKK activity; anterograde axon cargo transport; axon guidance; axon regeneration in the peripheral nervous system; axon transport of mitochondrion; cerebral cortex development; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; hippocampus development; innate immune response; insulin receptor signaling pathway; intermediate filament organization; intermediate filament polymerization and/or depolymerization; MAPKKK cascade; microtubule cytoskeleton organization and biogenesis; negative regulation of neuron apoptosis; nerve growth factor receptor signaling pathway; neurofilament bundle assembly; neuromuscular process controlling balance; positive regulation of axonogenesis; protein polymerization; Ras protein signal transduction; regulation of axon diameter; response to corticosterone stimulus; response to toxin; retrograde axon cargo transport; small GTPase mediated signal transduction; spinal cord development; synaptic transmission; vascular endothelial growth factor receptor signaling pathway. Disease: Charcot-marie-tooth Disease, Axonal, Type 2e; Charcot-marie-tooth Disease, Demyelinating, Type 1f

48-Strip-Wells

300 USD

96-Strip-Wells

415

5x96-Strip-Wells

1825

10x96-Strip-Wells

3425