ELISA/assay

Human Beta-arrestin-2 ELISA Kit

MBS765831

48-Strip-Wells

300 USD

ELISA Kit

Human Beta-arrestin-2 ELISA Kit

[Beta-arrestin-2]

[ARRb2 (Arrestin Beta 2)/ARRB2/ARB2/ARR2/BARR2/Beta-arrestin-2/Arrestin beta-2/arrestin 3/Arrestin beta-2/arrestin; beta 2/beta-arrestin-2]

[beta-arrestin-2 isoform 6; Beta-arrestin-2; beta-arrestin-2; arrestin, beta 2; Arrestin beta-2]

[ARRB2]

[ARRB2; ARRB2; ARB2; ARR2; BARR2; ARB2; ARR2]

ARRB2_HUMAN

Human

406

This assay has high sensitivity and excellent specificity for detection of ARRB2. No significant cross-reactivity or interference between ARRB2 and analogues was observed.

Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich ELISA, Double Antibody. Detection Range: 62.5-4000pg/ml. Sensitivity: 37.5pg/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ARRB2 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ARRB2 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- ARRB2 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- ARRB2 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the ARRB2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of ARRB2 can be calculated.

382545442

NP_001244260.1

NM_001257331.1

44,392 Da

ALK1 Signaling Events Pathway (137968); ARMS-mediated Activation Pathway (1269471); Activated NOTCH1 Transmits Signal To The Nucleus Pathway (1269536); Activation Of SMO Pathway (1269642); Arf6 Signaling Events Pathway (138034); Atypical NF-kappaB Pathway (137946); Axon Guidance Pathway (1270303); Beta-catenin Independent WNT Signaling Pathway (1269610); CXCR4-mediated Signaling Events Pathway (137910); Calcium Regulation In The Cardiac Cell Pathway (198906)

Members of arrestin/beta-arrestin protein family are thought to participate in agonist-mediated desensitization of G-protein-coupled receptors and cause specific dampening of cellular responses to stimuli such as hormones, neurotransmitters, or sensory signals. Arrestin beta 2, like arrestin beta 1, was shown to inhibit beta-adrenergic receptor function in vitro. It is expressed at high levels in the central nervous system and may play a role in the regulation of synaptic receptors. Besides the brain, a cDNA for arrestin beta 2 was isolated from thyroid gland, and thus it may also be involved in hormone-specific desensitization of TSH receptors. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2012]

ARRB2: a member of the arrestin/beta-arrestin protein family. These proteins participate in agonist-mediated desensitization of G-protein-coupled receptors. Acts as a cofactor in the beta-adrenergic receptor kinase (BARK) mediated desensitization of beta-adrenergic receptors. Expressed at high levels in the CNS and peripheral blood leukocytes. Protein type: Adaptor/scaffold. Chromosomal Location of Human Ortholog: 17p13. Cellular Component: basolateral plasma membrane; coated pit; cytoplasm; cytoplasmic vesicle; cytosol; dendritic spine; endocytic vesicle; nucleus; plasma membrane; postsynaptic density; postsynaptic membrane. Molecular Function: alpha-1A adrenergic receptor binding; alpha-1B adrenergic receptor binding; angiotensin receptor binding; D1 dopamine receptor binding; enzyme binding; follicle stimulating hormone receptor binding; G-protein-coupled receptor binding; low-density lipoprotein receptor binding; mitogen-activated protein kinase binding; platelet activating factor receptor binding; protein binding; protein complex binding; protein complex scaffold; protein domain specific binding; protein kinase B binding; receptor binding; type 1 angiotensin receptor binding; type 2A serotonin receptor binding; ubiquitin protein ligase binding. Biological Process: adult walking behavior; arrestin mediated desensitization of G-protein coupled receptor protein signaling pathway; blood coagulation; brain development; detection of temperature stimulus involved in sensory perception of pain; follicle-stimulating hormone signaling pathway; G-protein coupled receptor internalization; inhibition of NF-kappaB transcription factor; negative regulation of caspase activity; negative regulation of interleukin-1 beta production; negative regulation of interleukin-12 production; negative regulation of interleukin-6 production; negative regulation of natural killer cell mediated cytotoxicity; negative regulation of protein ubiquitination; negative regulation of toll-like receptor signaling pathway; negative regulation of tumor necrosis factor production; Notch signaling pathway; platelet activation; positive regulation of calcium ion transport; positive regulation of peptidyl-serine phosphorylation; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of protein kinase B signaling cascade; positive regulation of protein ubiquitination; positive regulation of receptor internalization; positive regulation of synaptic transmission, dopaminergic; proteasomal ubiquitin-dependent protein catabolic process; protein transport; protein ubiquitination; receptor internalization; transcription from RNA polymerase II promoter; transforming growth factor beta receptor signaling pathway

48-Strip-Wells

300 USD

96-Strip-Wells

415

5x96-Strip-Wells

1825

10x96-Strip-Wells

3425