ELISA/assay

Mouse Matrix Metalloproteinase 1 ELISA Kit

MBS765800

48-Strip-Wells

300 USD

ELISA Kit

Mouse Matrix Metalloproteinase 1 ELISA Kit

[Matrix Metalloproteinase 1]

[MMP-1 (Matrix metallopeptidase 1)/CLGmatrix metalloprotease 1/CLGN/Fibroblast collagenase/interstitial collagenase/matrix metalloproteinase 1 (interstitial collagenase)]

[matrix metalloproteinase 1, isoform L; CG4859 gene product from transcript CG4859-RI; Matrix metalloproteinase 1]

[MMP-1]

[Mmp1; CG4859; dm1-MMP; Dm1-MMP; DmelCG4859; dmmp1; dMMP1; l(2)k04809; Mmp 1; MMP-1; mmp1; MMp1; MMP1]

Mouse

512

This assay has high sensitivity and excellent specificity for detection of MMP-1. No significant cross-reactivity or interference between MMP-1 and analogues was observed.

Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids. Assay Type: Sandwich. Detection Range: 15.625-1000pg/ml. Sensitivity: <9.375pg/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level MMP-1 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level MMP-1 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-MMP-1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-MMP-1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the MMP-1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of MMP-1 can be calculated.

386768590

NP_001246499.1

NM_001259570.2

Activation Of Matrix Metalloproteinases Pathway (1328721); Collagen Degradation Pathway (1328722); Degradation Of The Extracellular Matrix Pathway (1328720); Extracellular Matrix Organization Pathway (1328708)

48-Strip-Wells

300 USD

96-Strip-Wells

415

5x96-Strip-Wells

1825

10x96-Strip-Wells

3425