catalog number :
MBS765748
products type :
ELISA Kit
products full name :
Mouse Melatonin ELISA Kit
products short name :
[Melatonin]
products name syn :
[MT (Melatonin)]
products gene name :
[MT]
specificity :
This assay has high sensitivity and excellent specificity for detection of IS. No significant cross-reactivity or interference between IS and analogues was observed.
storage stability :
Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids. Assay Type: Competitive. Detection Range: 31.25-2000ng/ml. Sensitivity: <18.75ng/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level IS were tested 20 times on one plate, respectively. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level IS were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
products description :
Principle of the Assay: This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with IS. During the reaction, IS in the sample or standard competes with a fixed amount of IS on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to IS. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin(SABC) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of IS in the samples is then determined by comparing the O.D. of the samples to the standard curve.
size4 :
10x96-Strip-Wells