catalog number :
MBS765686
products type :
ELISA Kit
products full name :
Human Free Prostate Specific Antigen ELISA Kit
products short name :
[Free Prostate Specific Antigen]
products name syn :
[fPSA/APS/KLK2A1/hK3/Kallikrein-3/Kallikrein-Related Peptidase 3/Gamma-seminoprotein]
products gene name :
[fPSA]
storage stability :
Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
image1 heading :
Typical Testing Data
image2 heading :
Typical Standard Curve
other info1 :
Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich ELISA, Double Antibody. Detection Range: 0.938-60ng/ml. Sensitivity: 0.563ng/ml
products description :
Principle of the Assay This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human fPSA antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human fPSA antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human fPSA amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human fPSA can be calculated.
ncbi acc num :
AKM49063.1
size4 :
10x96-Strip-Wells