Human Muellerian-inhibiting factor ELISA Kit | MyBioSource MBS765586 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Muellerian-inhibiting factor ELISA Kit

catalog :

MBS765586

quantity :

48-Strip-Wells

price :

300 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS765586

products type :

ELISA Kit

products full name :

Human Muellerian-inhibiting factor ELISA Kit

products short name :

[Muellerian-inhibiting factor]

products name syn :

[AMH/MIS/muellerian-inhibiting factor/Mullerian inhibiting factor//Anti-Muellerian hormone (AMH)/Muellerian-inhibiting substance (MIS)]

other names :

[muellerian-inhibiting factor; Muellerian-inhibiting factor; muellerian-inhibiting factor; anti-Mullerian hormone; Anti-Muellerian hormone; AMH; Muellerian-inhibiting substance; MIS]

products gene name :

[AMH]

other gene names :

[AMH; AMH; MIF; MIS; MIF; AMH; MIS]

uniprot entry name :

MIS_HUMAN

reactivity :

Human

sequence length :

560

specificity :

This assay has high sensitivity and excellent specificity for detection of AMH. No significant cross-reactivity or interference between AMH and analogues was observed.

storage stability :

Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, plasma, tissue homogenates and other biological fluids. Detection Range: 46.875-3000pg/ml. Sensitivity: <28.125pg/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level AMH were tested 20 times on one plate, respectively. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level AMH were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%

products description :

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- AMH antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- AMH antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the AMH amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of AMH can be calculated.

ncbi gi num :

157266298

ncbi acc num :

NP_000470.2

ncbi gb acc num :

NM_000479.3

ncbi mol weight :

59,195 Da

ncbi pathways :

ALK2 Signaling Events Pathway (137983); BMP Signaling Pathway (1084755); BMP Signaling Pathway (1108218); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Hippo Signaling Pathway (749777); Hippo Signaling Pathway (750388); TGF-beta Signaling Pathway (83064); TGF-beta Signaling Pathway (475); CAMP Signaling Pathway (1017634)

ncbi summary :

Anti-Mullerian hormone is a member of the transforming growth factor-beta gene family which mediates male sexual differentiation. Anti-Mullerian hormone causes the regression of Mullerian ducts which would otherwise differentiate into the uterus and fallopian tubes. Some mutations in the anti-Mullerian hormone result in persistent Mullerian duct syndrome. [provided by RefSeq, Jul 2008]

uniprot summary :

AMH: This glycoprotein, produced by the Sertoli cells of the testis, causes regression of the Muellerian duct. It is also able to inhibit the growth of tumors derived from tissues of Muellerian duct origin. Defects in AMH are the cause of persistent Muellerian duct syndrome type 1 (PMDS1). PMDS1 is a form of male pseudohermaphroditism characterized by a failure of Muellerian duct regression in otherwise normal males. Belongs to the TGF-beta family. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 19p13.3. Cellular Component: cytoplasm; extracellular region; extracellular space. Molecular Function: growth factor activity; hormone activity; receptor binding; transforming growth factor beta receptor binding. Biological Process: activation of NF-kappaB transcription factor; aging; cell-cell signaling; gonadal mesoderm development; Mullerian duct regression; preantral ovarian follicle growth; response to drug; response to organic cyclic substance; sex determination; sex differentiation; urogenital system development. Disease: Persistent Mullerian Duct Syndrome, Types I And Ii

size1 :

48-Strip-Wells

price1 :

300 USD

size2 :

96-Strip-Wells

price2 :

415

size3 :

5x96-Strip-Wells

price3 :

1825

size4 :

10x96-Strip-Wells

price4 :

3425

more info or order :

MyBioSource product webpage