Human Peptide YY ELISA Kit | MyBioSource MBS765494 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Peptide YY ELISA Kit

catalog :

MBS765494

quantity :

48-Strip-Wells

price :

300 USD

product information

catalog number :

MBS765494

products type :

ELISA Kit

products full name :

Human Peptide YY ELISA Kit

products short name :

[Peptide YY]

products name syn :

[PYY/Peptide YY/PYY-I/Peptide tyrosine tyrosine]

other names :

[peptide YY preproprotein; Peptide YY; peptide YY; peptide YY; PYY-II]

products gene name :

[PYY]

other gene names :

[PYY; PYY; PYY1; PYY-I; PYY]

uniprot entry name :

PYY_HUMAN

reactivity :

Human

sequence length :

specificity :

This assay has high sensitivity and excellent specificity for detection of PYY. No significant cross-reactivity or interference between PYY and analogues was observed.

storage stability :

Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: serum, plasma, tissue homogenates and other biological fluids. Assay Type: Quantitative Sandwich. Detection Range: 31.25-2000pg/ml. Sensitivity: <18.75pg/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level PYY were tested 20 times on one plate, respectively. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level PYY were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%

products description :

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- PYY antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- PYY antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the PYY amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of PYY can be calculated.

ncbi gi num :

300068956

ncbi acc num :

NP_004151.3

ncbi gb acc num :

NM_004160.5

ncbi mol weight :

10,360 Da

ncbi pathways :

Class A/1 (Rhodopsin-like Receptors) Pathway (1269545); G Alpha (i) Signalling Events Pathway (1269576); GPCR Downstream Signaling Pathway (1269574); GPCR Ligand Binding Pathway (1269544); Peptide Ligand-binding Receptors Pathway (1269546); Signal Transduction Pathway (1269379); Signaling By GPCR Pathway (1269543)

ncbi summary :

This gene encodes a member of the neuropeptide Y (NPY) family of peptides. The encoded preproprotein is proteolytically processed to generate two alternative peptide products that differ in length by three amino acids. These peptides, secreted by endocrine cells in the gut, exhibit different binding affinities for each of the neuropeptide Y receptors. Binding of the encoded peptides to these receptors mediates regulation of pancreatic secretion, gut mobility and energy homeostasis. Rare variations in this gene could increase susceptibility to obesity and elevated serum levels of the encoded peptides may be associated with anorexia nervosa. [provided by RefSeq, Feb 2016]

uniprot summary :

PYY: This gut peptide inhibits exocrine pancreatic secretion, has a vasoconstrictory action and inhibitis jejunal and colonic mobility. Belongs to the NPY family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 17q21.1. Cellular Component: extracellular region; extracellular space. Molecular Function: G-protein-coupled receptor binding; hormone activity; neuropeptide hormone activity; protein binding. Biological Process: cell motility; cell proliferation; cell-cell signaling; cytoskeleton organization and biogenesis; digestion; eating behavior; feeding behavior; G-protein coupled receptor protein signaling pathway; neuropeptide signaling pathway; regulation of appetite. Disease: Obesity

size1 :

48-Strip-Wells

price1 :

300 USD

size2 :

96-Strip-Wells

price2 :

415

size3 :

5x96-Strip-Wells

price3 :

1825

size4 :

10x96-Strip-Wells

price4 :

3425