ELISA/assay

Chicken Interleukin 2 ELISA Kit

MBS765452

48-Strip-Wells

300 USD

ELISA Kit

Chicken Interleukin 2 ELISA Kit

[Interleukin 2]

[interleukin-2; Interleukin-2; interleukin-2; interleukin 2; T-cell growth factor; TCGF; INN: Aldesleukin]

[IL-2]

[IL2; IL2; IL-2; TCGF; lymphokine; IL-2; TCGF]

IL2_HUMAN

Chicken

153

This assay has high sensitivity and excellent specificity for detection of IL-2. No significant cross-reactivity or interference between IL-2 and analogues was observed.

Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Samples: serum, plasma, tissue homogenates and other biological fluids. Assay Type: Quantitative Sandwich. Detection Range: 31.25-2000pg/ml. Sensitivity: <18.75pg/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level IL-2 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level IL-2 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- IL-2 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- IL-2 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IL-2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of IL-2 can be calculated.

28178861

NP_000577.2

NM_000586.3

17,628 Da

ARMS-mediated Activation Pathway (1269471); AhR Pathway (755436); Allograft Rejection Pathway (83123); Allograft Rejection Pathway (535); Autoimmune Thyroid Disease Pathway (83121); Autoimmune Thyroid Disease Pathway (533); Axon Guidance Pathway (1270303); Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway (137993); Calcium Signaling In The CD4+ TCR Pathway (137941); Chagas Disease (American Trypanosomiasis) Pathway (147809)

The protein encoded by this gene is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli. [provided by RefSeq, Jul 2008]

IL2: Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine- activated killer cells, natural killer cells, and glioma cells. A chromosomal aberration involving IL2 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with involves TNFRSF17. Belongs to the IL-2 family. Protein type: Oncoprotein; Secreted, signal peptide; Cytokine; Secreted. Chromosomal Location of Human Ortholog: 4q26-q27. Cellular Component: cytosol; extracellular region; extracellular space. Molecular Function: carbohydrate binding; cytokine activity; glycosphingolipid binding; growth factor activity; interleukin-2 receptor binding; kappa-type opioid receptor binding; kinase activator activity. Biological Process: activation of MAPKK activity; adaptive immune response; axon guidance; cell adhesion; cell-cell signaling; elevation of cytosolic calcium ion concentration; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; immune response; innate immune response; insulin receptor signaling pathway; MAPKKK cascade; natural killer cell activation; negative regulation of apoptosis; negative regulation of B cell apoptosis; negative regulation of heart contraction; negative regulation of inflammatory response; negative regulation of lymphocyte proliferation; negative regulation of protein amino acid phosphorylation; nerve growth factor receptor signaling pathway; positive regulation of activated T cell proliferation; positive regulation of B cell proliferation; positive regulation of cell growth; positive regulation of cell proliferation; positive regulation of immunoglobulin secretion; positive regulation of inflammatory response; positive regulation of interferon-gamma production; positive regulation of interleukin-17 production; positive regulation of isotype switching to IgG isotypes; positive regulation of regulatory T cell differentiation; positive regulation of tissue remodeling; positive regulation of transcription from RNA polymerase II promoter; positive regulation of tyrosine phosphorylation of Stat5 protein; protein kinase C activation; Ras protein signal transduction; regulation of T cell homeostatic proliferation; response to ethanol; small GTPase mediated signal transduction; T cell differentiation; vascular endothelial growth factor receptor signaling pathway

48-Strip-Wells

300 USD

96-Strip-Wells

415

5x96-Strip-Wells

1825

10x96-Strip-Wells

3425