ELISA/assay

Rat S100 Calcium Binding Protein B ELISA Kit

MBS765442

48-Strip-Wells

300 USD

ELISA Kit

Rat S100 Calcium Binding Protein B ELISA Kit

[S100 Calcium Binding Protein B]

[S100B (Protein S100-B)/S100/S100 beta/S100 calcium binding protein B/S100 calcium-binding protein B/S100 calcium-binding protein; beta (neural)/S-100 calcium-binding protein; beta chain; 10/S-100 protein beta chain/S-100 protein subunit beta/S100beta]

[S100 calcium binding protein B; Protein S100-B; protein S100-B; S100 calcium binding protein B; S-100 protein beta chain; S-100 protein subunit beta; S100 calcium-binding protein B]

[S100B]

[S100B; S100B; NEF; S100; S100-B; S100beta]

S100B_HUMAN

Rat

92

This assay has high sensitivity and excellent specificity for detection of S100B. No significant cross-reactivity or interference between S100B and analogues was observed.

Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, plasma, tissue homogenates and other biological fluids. Detection Range: 62.5-4000pg/ml. Sensitivity: <37.5pg/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level S100B were tested 20 times on one plate, respectively. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level S100B were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- S100B antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- S100B antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the S100B amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of S100B can be calculated.

12804681

AAH01766.1

10,713 Da

Activated TLR4 Signalling Pathway (1269236); Advanced Glycosylation Endproduct Receptor Signaling Pathway (1269258); Cytosolic Sensors Of Pathogen-associated DNA Pathway (1269268); DEx/H-box Helicases Activate Type I IFN And Inflammatory Cytokines Production Pathway (1269276); Immune System Pathway (1269170); Innate Immune System Pathway (1269203); MyD88 Cascade Initiated On Plasma Membrane Pathway (1269207); MyD88 Dependent Cascade Initiated On Endosome Pathway (1269228); MyD88-independent TLR3/TLR4 Cascade Pathway (1269220); MyD88:Mal Cascade Initiated On Plasma Membrane Pathway (1269237)

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21; however, this gene is located at 21q22.3. This protein may function in Neurite extension, proliferation of melanoma cells, stimulation of Ca2+ fluxes, inhibition of PKC-mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly. Chromosomal rearrangements and altered expression of this gene have been implicated in several neurological, neoplastic, and other types of diseases, including Alzheimer's disease, Down's syndrome, epilepsy, amyotrophic lateral sclerosis, melanoma, and type I diabetes. [provided by RefSeq, Jul 2008]

S100B: Weakly binds calcium but binds zinc very tightly- distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling. Dimer of either two alpha chains, or two beta chains, or one alpha and one beta chain. The S100B dimer binds two molecules of STK38. Interacts with AGER. The S100B dimer interacts with two molecules of CAPZA1. Although predominant among the water-soluble brain proteins, S100 is also found in a variety of other tissues. Belongs to the S-101 family. Protein type: Calcium-binding. Chromosomal Location of Human Ortholog: 21q22.3. Cellular Component: cell soma; cytoplasm; extracellular region; extracellular space; intracellular membrane-bound organelle; nucleus; perinuclear region of cytoplasm; ruffle. Molecular Function: calcium ion binding; calcium-dependent protein binding; identical protein binding; protein binding; protein homodimerization activity; RAGE receptor binding; tau protein binding; zinc ion binding. Biological Process: astrocyte differentiation; axonogenesis; cell proliferation; central nervous system development; innate immune response; learning and/or memory; memory; positive regulation of apoptosis; positive regulation of cell proliferation; positive regulation of I-kappaB kinase/NF-kappaB cascade; regulation of cell shape; regulation of neuronal synaptic plasticity; response to glucocorticoid stimulus; response to methylmercury

48-Strip-Wells

300 USD

96-Strip-Wells

415

5x96-Strip-Wells

1825

10x96-Strip-Wells

3425