ELISA/assay

Human Integrin beta-3 ELISA Kit

MBS765397

48-Strip-Wells

300 USD

ELISA Kit

Human Integrin beta-3 ELISA Kit

[Integrin beta-3]

[ITGB3/Integrin beta-3/Platelet membrane glycoprotein IIIa/GPIIIa/GP3A/CD61/INGRB3/ITG B3]

[integrin beta-3; Integrin beta-3; integrin beta-3; integrin subunit beta 3; Platelet membrane glycoprotein IIIa; GPIIIa; CD_antigen: CD61]

[ITGB3]

[ITGB3; ITGB3; GT; CD61; GP3A; BDPLT2; GPIIIa; BDPLT16; GP3A; GPIIIa]

ITB3_HUMAN

Human

788

This assay has high sensitivity and excellent specificity for detection of ITGB3. No significant cross-reactivity or interference between ITGB3 and analogues was observed.

Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Samples: serum, plasma, tissue homogenates and other biological fluids. Assay Type: Quantitative Sandwich. Detection Range: 78-5000pg/ml. Sensitivity: < 46.9pg/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ITGB3were tested 20 times on one plate, respectively. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ITGB3were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- ITGB3antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- ITGB3 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the ITGB3 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of ITGB3 can be calculated.

47078292

NP_000203.2

NM_000212.2

86,694 Da

ARMS-mediated Activation Pathway (1269471); Arf6 Signaling Events Pathway (138034); Arrhythmogenic Right Ventricular Cardiomyopathy Pathway (672454); Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway (117293); Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway (116129); Axon Guidance Pathway (1270303); Cell Surface Interactions At The Vascular Wall Pathway (1269373); Cytokine Signaling In Immune System Pathway (1269310); DAP12 Interactions Pathway (1269283); DAP12 Signaling Pathway (1269284)

The ITGB3 protein product is the integrin beta chain beta 3. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. Integrin beta 3 is found along with the alpha IIb chain in platelets. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling. [provided by RefSeq, Jul 2008]

ITGB3: integrin alpha-V/beta-3 is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin alpha-IIB/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. Integrins alpha-IIB/beta-3 and alpha-V/beta-3 recognize the sequence R-G-D in a wide array of ligands. Integrin alpha-IIB/beta-3 recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha- IIB/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial surface. Protein type: Membrane protein, integral; Cell adhesion; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 17q21.32. Cellular Component: cell surface; filopodium membrane; focal adhesion; integral to plasma membrane; integrin complex; melanosome; microvillus membrane; nucleus; plasma membrane; platelet alpha granule membrane; receptor complex. Molecular Function: cell adhesion molecule binding; enzyme binding; extracellular matrix binding; fibronectin binding; identical protein binding; platelet-derived growth factor receptor binding; protease binding; protein binding; protein disulfide isomerase activity; vascular endothelial growth factor receptor 2 binding; viral receptor activity. Biological Process: activation of protein kinase activity; angiogenesis involved in wound healing; axon guidance; blood coagulation; cell adhesion; cell growth; cell migration; cell-matrix adhesion; cell-substrate adhesion; cell-substrate junction assembly; entry of virus into host cell; extracellular matrix organization and biogenesis; heterotypic cell-cell adhesion; integrin-mediated signaling pathway; leukocyte migration; mesodermal cell differentiation; negative chemotaxis; negative regulation of lipid transport; negative regulation of lipoprotein metabolic process; negative regulation of low-density lipoprotein receptor biosynthetic process; platelet activation; platelet degranulation; positive regulation of endothelial cell proliferation; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of protein amino acid phosphorylation; positive regulation of vascular endothelial growth factor receptor signaling pathway; protein folding; regulation of bone resorption; smooth muscle cell migration; tube development; vascular endothelial growth factor receptor signaling pathway; wound healing. Disease: Bleeding Disorder, Platelet-type, 16; Glanzmann Thrombasthenia; Myocardial Infarction, Susceptibility To

48-Strip-Wells

300 USD

96-Strip-Wells

415

5x96-Strip-Wells

1825

10x96-Strip-Wells

3425