product summary
Loading...
company name :
MyBioSource
product type :
ELISA/assay
product name :
Mouse Free Thyroxine ELISA Kit
catalog :
MBS765283
quantity :
48-Strip-Wells
price :
300 USD
more info or order :
image
image 1 :
product information
catalog number :
MBS765283
products type :
ELISA Kit
products full name :
Mouse Free Thyroxine ELISA Kit
products short name :
[Free Thyroxine]
products name syn :
[fT4 (Free Thyroxine)]
other names :
[FT4; FT4; uncharacterized protein LOC100803719; FT4]
products gene name :
[fT4]
other gene names :
[FTL8; FTL8; FT4]
uniprot entry name :
G7Z0A8_SOYBN
reactivity :
Mouse
sequence length :
171
specificity :
This assay has high sensitivity and excellent specificity for detection of fT3. No significant cross-reactivity or interference between fT3 and analogues is observed.
storage stability :
Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids. Assay Type: Competitive. Detection Range: 0.781-50pg/ml. Sensitivity: <0.469pg/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level fT3 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level fT3 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
products description :
Principle of the Assay: This kit was based on Competitive-ELISA detection method. The microtiter plate provided in this kit has been pre-coated with fT3. During the reaction, fT3 in the sample or standard competes with a fixed amount of fT3 on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to fT3. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of fT3 in the samples is then determined by comparing the O D of the samples to the standard curve.
ncbi gi num :
751155074
ncbi acc num :
AJF40162.1
ncbi mol weight :
19,408 Da
ncbi pathways :
Circadian Rhythm - Plant Pathway (552811); Circadian Rhythm - Plant Pathway (95644)
size1 :
48-Strip-Wells
price1 :
300 USD
size2 :
96-Strip-Wells
price2 :
415
size3 :
5x96-Strip-Wells
price3 :
1825
size4 :
10x96-Strip-Wells
price4 :
3425
more info or order :
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.
"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."
Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...
"MyBioSource offers the best products at unbeatable prices."
Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.
Would you like to receive email and e-newsletter from MyBioSource about new products, special offers and events? Please click here to join our Mailing List!