catalog number :
MBS765277
products type :
ELISA Kit
products full name :
Human Cytotoxic T-lymphocyte protein 4 ELISA Kit
products short name :
[Cytotoxic T-lymphocyte protein 4]
products name syn :
[CTLA4 (Cytotoxic T Lymphocyte Associated Antigen 4)/CD152/CD/CD152 antigen/CD28/celiac disease 3/CELIAC3/CTLA-4/cytotoxic T-lymphocyte protein 4/Cytotoxic T-lymphocyte-associated antigen 4/cytotoxic T-lymphocyte-associated protein 4/GRD4/GSE/ICOS]
other names :
[cytotoxic T-lymphocyte protein 4; cytotoxic T-lymphocyte protein 4; cytotoxic T-lymphocyte-associated protein 4]
products gene name :
[CTLA4]
other gene names :
[Ctla4; Cd152; CTLA-4; sCTLA4]
specificity :
This assay has high sensitivity and excellent specificity for detection of Ctgf. No significant cross-reactivity or interference between Ctgf and analogues was observed.
storage stability :
Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids. Assay Type: Sandwich. Detection Range: 15.6-1000pg/ml. Sensitivity: <9.375pg/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ctgf were tested 20 times on one plate, respectively. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ctgf were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
products description :
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Ctgf antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Ctgf antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Ctgf amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Ctgf can be calculated.
ncbi acc num :
NP_113862.1
ncbi gb acc num :
NM_031674.1
ncbi pathways :
Adaptive Immune System Pathway (1332755); Autoimmune Thyroid Disease Pathway (83509); Autoimmune Thyroid Disease Pathway (533); CTLA4 Inhibitory Signaling Pathway (1332765); Cell Adhesion Molecules (CAMs) Pathway (83461); Cell Adhesion Molecules (CAMs) Pathway (480); Costimulation By The CD28 Family Pathway (1332761); Immune System Pathway (1332754); Rheumatoid Arthritis Pathway (200306); Rheumatoid Arthritis Pathway (200269)
ncbi summary :
immunoregulatory receptor expressed on the surface of T and B lymphocytes [RGD, Feb 2006]
size4 :
10x96-Strip-Wells