Human Insulin-Like Growth Factor 1 ELISA Kit | MyBioSource MBS765203 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Insulin-Like Growth Factor 1 ELISA Kit

catalog :

MBS765203

quantity :

48-Strip-Wells

price :

300 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS765203

products type :

ELISA Kit

products full name :

Human Insulin-Like Growth Factor 1 ELISA Kit

products short name :

[Insulin-Like Growth Factor 1]

products name syn :

[IGF-1/IGF1/IGFI/IGF-I/IGF1A/IGF-IA/IGF-IB/MGF/Somatomedin C]

other names :

[insulin-like growth factor I preproprotein; Insulin-like growth factor I; insulin-like growth factor I; Somatomedin]

products gene name :

[IGF-1]

other gene names :

[IGF1; IGF1; IGF-1; IGF-I; IGF-I]

uniprot entry name :

IGF1_BOVIN

reactivity :

Human

sequence length :

154

specificity :

This assay has high sensitivity and excellent specificity for detection of IGF-1. No significant cross-reactivity or interference between IGF-1 and analogues was observed.

storage stability :

Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids. Assay Type: Sandwich. Detection Range: 1.563-100ng/ml. Sensitivity: <0.938ng/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level IGF-1 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level IGF-1 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%

products description :

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-IGF-1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-IGF-1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IGF-1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of IGF-1 can be calculated.

ncbi gi num :

118150782

ncbi acc num :

NP_001071296.1

ncbi gb acc num :

NM_001077828.1

ncbi mol weight :

17,066 Da

ncbi pathways :

AMPK Signaling Pathway (989258); AMPK Signaling Pathway (992181); Adipogenesis Pathway (920913); Aldosterone-regulated Sodium Reabsorption Pathway (130625); Aldosterone-regulated Sodium Reabsorption Pathway (130590); Androgen Receptor Signaling Pathway (198553); Apoptosis Pathway (198572); Dilated Cardiomyopathy Pathway (121480); Dilated Cardiomyopathy Pathway (121285); Endochondral Ossification Pathway (920938)

uniprot summary :

The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation. Ca2+-dependent exocytosis of IGF1 is required for sensory perception of smell in the olfactory bulb. Acts as a ligand for IGF1R. Binds to the alpha subunit of IGF1R, leading to the activation of the intrinsic tyrosine kinase activity which autophosphorylates tyrosine residues in the beta subunit thus initiatiating a cascade of down-stream signaling events leading to activation of the PI3K-AKT/PKB and the Ras-MAPK pathways. Binds to integrins ITGAV:ITGB3 and ITGA6:ITGB4. Its binding to integrins and subsequent ternary complex formation with integrins and IGFR1 are essential for IGF1 signaling. Induces the phosphorylation and activation of IGFR1, MAPK3/ERK1, MAPK1/ERK2 and AKT1 ().

size1 :

48-Strip-Wells

price1 :

300 USD

size2 :

96-Strip-Wells

price2 :

415

size3 :

5x96-Strip-Wells

price3 :

1825

size4 :

10x96-Strip-Wells

price4 :

3425

more info or order :

MyBioSource product webpage