ELISA/assay

Mouse Transthyretin ELISA Kit

MBS765196

48-Strip-Wells

300 USD

ELISA Kit

Mouse Transthyretin ELISA Kit

[Transthyretin]

[TTR/PALB/TBPA/ATTR/Prealbumin/HsT2651/prealbumin; amyloidosis type I/thyroxine-binding prealbumin]

[transthyretin; Transthyretin; transthyretin; transthyretin; ATTR; Prealbumin; TBPA]

[TTR]

[TTR; TTR; CTS; CTS1; PALB; TBPA; HEL111; HsT2651; PALB]

TTHY_HUMAN

Mouse

147

This assay has high sensitivity and excellent specificity for detection of TTR. No significant cross-reactivity or interference between TTR and analogues was observed.

Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids. Assay Type: Sandwich. Detection Range: 15.625-1000ng/ml. Sensitivity: <9.375ng/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level TTR were tested 20 times on one plate, respectively. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level TTR were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-TTR antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-TTR antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the TTR amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of TTR can be calculated.

4507725

NP_000362.1

NM_000371.3

15,887 Da

Amyloid Fiber Formation Pathway (1269169); Disease Pathway (1268854); Diseases Associated With Visual Transduction Pathway (1268929); Diseases Of Signal Transduction Pathway (1268855); Extracellular Matrix Organization Pathway (1270244); FOXA2 And FOXA3 Transcription Factor Networks Pathway (137911); Metabolism Pathway (1269956); Metabolism Of Fat-soluble Vitamins Pathway (1339147); Metabolism Of Proteins Pathway (1268677); Metabolism Of Vitamins And Cofactors Pathway (1270144)

This gene encodes transthyretin, one of the three prealbumins including alpha-1-antitrypsin, transthyretin and orosomucoid. Transthyretin is a carrier protein; it transports thyroid hormones in the plasma and cerebrospinal fluid, and also transports retinol (vitamin A) in the plasma. The protein consists of a tetramer of identical subunits. More than 80 different mutations in this gene have been reported; most mutations are related to amyloid deposition, affecting predominantly peripheral nerve and/or the heart, and a small portion of the gene mutations is non-amyloidogenic. The diseases caused by mutations include amyloidotic polyneuropathy, euthyroid hyperthyroxinaemia, amyloidotic vitreous opacities, cardiomyopathy, oculoleptomeningeal amyloidosis, meningocerebrovascular amyloidosis, carpal tunnel syndrome, etc. [provided by RefSeq, Jan 2009]

TTR: Thyroid hormone-binding protein. Probably transports thyroxine from the bloodstream to the brain. Defects in TTR are the cause of amyloidosis transthyretin-related (AMYL-TTR). A hereditary generalized amyloidosis due to transthyretin amyloid deposition. Protein fibrils can form in different tissues leading to amyloid polyneuropathies, amyloidotic cardiomyopathy, carpal tunnel syndrome, systemic senile amyloidosis. The disease includes leptomeningeal amyloidosis that is characterized by primary involvement of the central nervous system. Neuropathologic examination shows amyloid in the walls of leptomeningeal vessels, in pia arachnoid, and subpial deposits. Some patients also develop vitreous amyloid deposition that leads to visual impairment (oculoleptomeningeal amyloidosis). Clinical features include seizures, stroke-like episodes, dementia, psychomotor deterioration, variable amyloid deposition in the vitreous humor. Defects in TTR are a cause of hyperthyroxinemia dystransthyretinemic euthyroidal (HTDE). It is a condition characterized by elevation of total and free thyroxine in healthy, euthyroid persons without detectable binding protein abnormalities. Defects in TTR are a cause of carpal tunnel syndrome type 1 (CTS1). It is a condition characterized by entrapment of the median nerve within the carpal tunnel. Symptoms include burning pain and paresthesias involving the ventral surface of the hand and fingers which may radiate proximally. Impairment of sensation in the distribution of the median nerve and thenar muscle atrophy may occur. This condition may be associated with repetitive occupational trauma, wrist injuries, amyloid neuropathies, rheumatoid arthritis. Belongs to the transthyretin family. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 18q12.1. Cellular Component: cytoplasm; extracellular region; extracellular space; protein complex. Molecular Function: hormone activity; hormone binding; identical protein binding; protein binding; protein heterodimerization activity. Biological Process: cellular protein metabolic process; extracellular matrix organization and biogenesis; fat-soluble vitamin metabolic process; phototransduction, visible light; retinoid metabolic process; retinol metabolic process; transport; vitamin metabolic process. Disease: Amyloidosis, Hereditary, Transthyretin-related; Carpal Tunnel Syndrome; Hyperthyroxinemia, Dystransthyretinemic

48-Strip-Wells

300 USD

96-Strip-Wells

415

5x96-Strip-Wells

1825

10x96-Strip-Wells

3425