ELISA/assay

Mouse Lymphotoxin-alpha ELISA Kit

MBS765171

48-Strip-Wells

300 USD

ELISA Kit

Mouse Lymphotoxin-alpha ELISA Kit

[Lymphotoxin-alpha]

[Lta/tnfb/TNF-beta/TNFSF1/TNFSF1B/LT/LT-alpha/lymphotoxin alpha (TNF superfamily; member 1)/TNFBlymphotoxin-alpha/TNFSF1TNF-beta/tumor necrosis factor beta/Tumor necrosis factor ligand superfamily member 1]

[lymphotoxin-alpha; Lymphotoxin-alpha; lymphotoxin-alpha; lymphotoxin alpha; TNF-beta; Tumor necrosis factor ligand superfamily member 1]

[Lta]

[LTA; LTA; LT; TNFB; TNFSF1; TNLG1E; TNFB; TNFSF1; LT-alpha]

TNFB_HUMAN

Mouse

205

This assay has high sensitivity and excellent specificity for detection ofLPS IgG. No significant cross-reactivity or interference betweenLPS IgGand analogues was observed.

Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Tissue Homogenatesand Other Biological Fluids. Assay Type: Sandwich. Detection Range: 1.563-100ng/ml. Sensitivity: <0.938ng/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high levelLPS IgGwere tested 20 times on one plate, respectively. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high levelLPS IgGwere tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-LPS IgGantibody was pre-coated onto 96-well plates. And the biotin conjugated anti-LPS IgG antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the LPS IgG amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of LPS IgG can be calculated.

229092381

NP_001153212.1

NM_001159740.2

22,297 Da

Apoptosis Pathway (198797); Cytokine Signaling In Immune System Pathway (1269310); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); HTLV-I Infection Pathway (373901); HTLV-I Infection Pathway (373889); Herpes Simplex Infection Pathway (377873); Herpes Simplex Infection Pathway (377865); IL2 Signaling Events Mediated By STAT5 Pathway (137988); IL4-mediated Signaling Events Pathway (137933)

The encoded protein, a member of the tumor necrosis factor family, is a cytokine produced by lymphocytes. The protein is highly inducible, secreted, and forms heterotrimers with lymphotoxin-beta which anchor lymphotoxin-alpha to the cell surface. This protein also mediates a large variety of inflammatory, immunostimulatory, and antiviral responses, is involved in the formation of secondary lymphoid organs during development and plays a role in apoptosis. Genetic variations in this gene are associated with susceptibility to leprosy type 4, myocardial infarction, non-Hodgkin's lymphoma, and psoriatic arthritis. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Jul 2012]

LTA: Cytokine that in its homotrimeric form binds to TNFRSF1A/TNFR1, TNFRSF1B/TNFBR and TNFRSF14/HVEM. In its heterotrimeric form with LTB binds to TNFRSF3/LTBR. Lymphotoxin is produced by lymphocytes and cytotoxic for a wide range of tumor cells in vitro and in vivo. Genetic variations in LTA are a cause of susceptibility psoriatic arthritis (PSORAS). PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis). Belongs to the tumor necrosis factor family. Protein type: Secreted; Secreted, signal peptide; Apoptosis; Cytokine. Chromosomal Location of Human Ortholog: 6p21.3. Cellular Component: extracellular space; plasma membrane. Molecular Function: cytokine activity; receptor binding; tumor necrosis factor receptor binding. Biological Process: apoptosis; cell-cell signaling; defense response to Gram-positive bacterium; humoral immune response; lymph node development; negative regulation of fibroblast proliferation; positive regulation of apoptosis; positive regulation of chronic inflammatory response to antigenic stimulus; positive regulation of humoral immune response mediated by circulating immunoglobulin; positive regulation of interferon-gamma production; response to drug; response to hypoxia; response to lipopolysaccharide; response to nutrient; signal transduction; tumor necrosis factor-mediated signaling pathway. Disease: Leprosy, Susceptibility To, 4; Myocardial Infarction, Susceptibility To; Psoriatic Arthritis, Susceptibility To

48-Strip-Wells

300 USD

96-Strip-Wells

415

5x96-Strip-Wells

1825

10x96-Strip-Wells

3425