Human Nitric oxide synthase, endothelial ELISA Kit | MyBioSource MBS765164 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Nitric oxide synthase, endothelial ELISA Kit

catalog :

MBS765164

quantity :

48-Strip-Wells

price :

300 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS765164

products type :

ELISA Kit

products full name :

Human Nitric oxide synthase, endothelial ELISA Kit

products short name :

[Nitric oxide synthase, endothelial]

products name syn :

[NOS3/eNOS/Constitutive NOS/ECNOS/endothelial/Endothelial NOS/eNOS/cNOS/nitric oxide synthase 3 (endothelial cell)/NOS type III/NOSIII]

other names :

[nitric oxide synthase, endothelial; Nitric oxide synthase, endothelial; nitric oxide synthase, endothelial; Constitutive NOS; cNOS; EC-NOS; Endothelial NOS; eNOS; NOS type III; NOSIII]

products gene name :

[NOS3/eNOS]

other gene names :

[NOS3; NOS3; cNOS; eNOS; NOSIII]

uniprot entry name :

NOS3_BOVIN

reactivity :

Human

sequence length :

1205

specificity :

This assay has high sensitivity and excellent specificity for detection of NOS3/eNOS. No significant cross-reactivity or interference between NOS3/eNOS and analogues was observed.

storage stability :

Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich ELISA, Double Antibody. Detection Range: 62.5-4000pg/ml. Sensitivity: 37.5pg/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level NOS3/eNOS were tested 20 times on one plate, respectively. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level NOS3/eNOS were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%

products description :

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- NOS3/eNOS antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- NOS3/eNOS antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the NOS3/eNOS amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of NOS3/eNOS can be calculated.

ncbi gi num :

317008623

ncbi acc num :

NP_851380.2

ncbi gb acc num :

NM_181037.3

ncbi mol weight :

133,287 Da

ncbi pathways :

ACE Inhibitor Pathway (198563); AGE-RAGE Signaling Pathway In Diabetic Complications (1319849); AGE-RAGE Signaling Pathway In Diabetic Complications (1319775); Arginine And Proline Metabolism Pathway (84202); Arginine And Proline Metabolism Pathway (323); Arginine Biosynthesis Pathway (84189); Arginine Biosynthesis Pathway (306); Calcium Signaling Pathway (84289); Calcium Signaling Pathway (459); Estrogen Signaling Pathway (799179)

uniprot summary :

eNOS: endothelial constitutive nitric oxide synthase. Synthesizes nitric oxide (NO) from arginine and oxygen, which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. Induced in humans by caloric restriction. Stimulated by calcium/calmodulin. Inhibited by NOSIP and NOSTRIN. Protein type: Oxidoreductase; Amino Acid Metabolism - arginine and proline; EC 1.14.13.39. Cellular Component: caveola; cytoskeleton; Golgi apparatus. Molecular Function: calmodulin binding; FAD binding; FMN binding; heme binding; iron ion binding; NADP binding; nitric-oxide synthase activity. Biological Process: arginine catabolic process; blood coagulation; mitochondrion organization and biogenesis; negative regulation of blood pressure; nitric oxide biosynthetic process; nitric oxide mediated signal transduction; positive regulation of guanylate cyclase activity

size1 :

48-Strip-Wells

price1 :

300 USD

size2 :

96-Strip-Wells

price2 :

415

size3 :

5x96-Strip-Wells

price3 :

1825

size4 :

10x96-Strip-Wells

price4 :

3425

more info or order :

MyBioSource product webpage