ELISA/assay

Mouse Beta-defensin 2 ELISA Kit

MBS765150

48-Strip-Wells

300 USD

ELISA Kit

Mouse Beta-defensin 2 ELISA Kit

[Beta-defensin 2]

[Defb2/BD-2/DEFB2/DEFB4A/DEFB4B/HBD-2/SAP-1/Beta-defensin 2/DEFB-2/DEFB2DEFB102/DEFB4beta-defensin 4A/Defensin; beta 2Skin-antimicrobial peptide 1/defensin; beta 4/defensin; beta 4A/HBD-2/hBD-2/SAP1BD-2]

[beta-defensin 2; Beta-defensin 4A; beta-defensin 4A; defensin beta 4A; Beta-defensin 2; BD-2; hBD-2; Defensin, beta 2; Skin-antimicrobial peptide 1; SAP1]

[Defb2]

[DEFB4A; DEFB4A; BD-2; SAP1; DEFB2; DEFB4; HBD-2; DEFB-2; DEFB102; DEFB102; DEFB2; DEFB4; BD-2; hBD-2; SAP1]

DFB4A_HUMAN

Mouse

64

This assay has high sensitivity and excellent specificity for detection of DA. No significant crossreactivity or interference between DA and analogues is observed.

Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids. Assay Type: Competitive. Detection Range: 1.56-100ng/ml. Sensitivity: <0.938ng/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level DA were tested 20 times on one plate, respectively. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level DA were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%

Principle of the Assay: This kit was based on Competitive-ELISA detection method. The microtiter plate provided in this kit has been pre-coated with DA. During the reaction, DA in the sample or standard competes with a fixed amount of DA on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to DA. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of DA in the samples is then determined by comparing the O D of the samples to the standard curve.

3510600

AAC33549.1

7,038 Da

Beta Defensins Pathway (1269267); Defensins Pathway (1269265); Immune System Pathway (1269170); Innate Immune System Pathway (1269203)

Defensins form a family of microbicidal and cytotoxic peptides made by neutrophils. Members of the defensin family are highly similar in protein sequence. This gene encodes defensin, beta 4, an antibiotic peptide which is locally regulated by inflammation. [provided by RefSeq, Jul 2008]

defensin, beta 2: Has antibacterial activity (Potential). Belongs to the beta-defensin family. LAP/TAP subfamily. Protein type: Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 8p23.1. Cellular Component: extracellular region; Golgi lumen. Biological Process: chemotaxis; defense response to bacterium; G-protein coupled receptor protein signaling pathway; immune response; innate immune response

48-Strip-Wells

300 USD

96-Strip-Wells

415

5x96-Strip-Wells

1825

10x96-Strip-Wells

3425