ELISA/assay

Human Alpha-amylase 1 ELISA Kit

MBS765071

48-Strip-Wells

300 USD

ELISA Kit

Human Alpha-amylase 1 ELISA Kit

[Alpha-amylase 1]

[AMY1/Alpha-amylase 1/Amylase Alpha 1; Salivary/1,4-alpha-D-glucan glucanohydrolase 1/Salivary alpha-amylase]

[alpha-amylase 1; Alpha-amylase 1; alpha-amylase 1; amylase, alpha 1A (salivary); 1,4-alpha-D-glucan glucanohydrolase 1; Salivary alpha-amylase]

[AMY1]

[AMY1A; AMY1A; AMY1; AMY1]

AMY1_HUMAN

Human

511

This assay has high sensitivity and excellent specificity for detection of ALT. No significant cross-reactivity or interference between ALT and analogues was observed.

Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids. Assay Type: Sandwich. Detection Range: 0.156-10ng/ml. Sensitivity: <0.094ng/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ALT were tested 20 times on one plate, respectively. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ALT were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-ALT antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-ALT antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the ALT amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of ALT can be calculated.

40254482

NP_004029.2

NM_004038.3

57,768 Da

Carbohydrate Digestion And Absorption Pathway (170720); Carbohydrate Digestion And Absorption Pathway (170654); Digestion Of Dietary Carbohydrate Pathway (1269958); Metabolic Pathways (132956); Metabolism Pathway (1269956); Metabolism Of Carbohydrates Pathway (1269957); Salivary Secretion Pathway (153376); Salivary Secretion Pathway (153352); Starch And Sucrose Metabolism Pathway (82974); Starch And Sucrose Metabolism Pathway (344)

Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. The human genome has a cluster of several amylase genes that are expressed at high levels in either salivary gland or pancreas. This gene encodes an amylase isoenzyme produced by the salivary gland. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008]

AMY1: Monomer. Belongs to the glycosyl hydrolase 13 family. Protein type: Carbohydrate Metabolism - starch and sucrose; EC 3.2.1.1; Hydrolase; Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 1p21. Cellular Component: extracellular space. Molecular Function: protein binding. Biological Process: digestion

48-Strip-Wells

300 USD

96-Strip-Wells

415

5x96-Strip-Wells

1825

10x96-Strip-Wells

3425