Human Glial fibrillary acidic protein ELISA Kit | MyBioSource MBS764819 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Glial fibrillary acidic protein ELISA Kit

catalog :

MBS764819

quantity :

48-Strip-Wells

price :

300 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS764819

products type :

ELISA Kit

products full name :

Human Glial fibrillary acidic protein ELISA Kit

products short name :

[Glial fibrillary acidic protein]

products name syn :

[GFAP/FLJ45472/glial fibrillary acidic protein]

other names :

[glial fibrillary acidic protein; Glial fibrillary acidic protein; glial fibrillary acidic protein; glial fibrillary acidic protein]

products gene name :

[GFAP]

other gene names :

[GFAP; GFAP; ALXDRD; GFAP]

uniprot entry name :

GFAP_HUMAN

reactivity :

Human

sequence length :

432

specificity :

This assay has high sensitivity and excellent specificity for detection of GDF7. No significant cross-reactivity or interference between GDF7 and analogues was observed.

storage stability :

Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids. Assay Type: Sandwich. Detection Range: 62.5-4000pg/ml. Sensitivity: <37.5pg/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level GDF7 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level GDF7 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%

products description :

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-GDF7 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-GDF7 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the GDF7 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of GDF7 can be calculated.

ncbi gi num :

251802

ncbi acc num :

AAB22581.1

ncbi mol weight :

49,508 Da

ncbi pathways :

Jak-STAT Signaling Pathway (83077); Jak-STAT Signaling Pathway (488); Neural Crest Differentiation Pathway (672460); Nuclear Signaling By ERBB4 Pathway (1269499); Signal Transduction Pathway (1269379); Signaling By ERBB4 Pathway (1269496); Spinal Cord Injury Pathway (739007)

ncbi summary :

This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008]

uniprot summary :

GFAP: a class-III intermediate filament protein. A cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. An additional transcript variant isoform has been described, but its full length sequence has not been determined. Protein type: Cytoskeletal. Chromosomal Location of Human Ortholog: 17q21. Cellular Component: cytoplasm; cytosol; intermediate filament; intermediate filament cytoskeleton; lysosome; membrane; myelin sheath. Molecular Function: integrin binding; kinase binding; protein binding; structural constituent of cytoskeleton. Biological Process: astrocyte development; Bergmann glial cell differentiation; extracellular matrix organization and biogenesis; intermediate filament organization; neurite regeneration; regulation of neurotransmitter uptake; regulation of protein complex assembly; response to wounding. Disease: Alexander Disease

size1 :

48-Strip-Wells

price1 :

300 USD

size2 :

96-Strip-Wells

price2 :

415

size3 :

5x96-Strip-Wells

price3 :

1825

size4 :

10x96-Strip-Wells

price4 :

3425

more info or order :

MyBioSource product webpage