ELISA/assay

Rat Follicle Stimulating Hormone ELISA Kit

MBS764815

48-Strip-Wells

300 USD

ELISA Kit

Rat Follicle Stimulating Hormone ELISA Kit

[Follicle Stimulating Hormone]

[FSH (Follicle-Stimulating Hormone)]

[follicle-stimulating hormone receptor isoform 2; Follicle-stimulating hormone receptor; follicle-stimulating hormone receptor; follicle stimulating hormone receptor; Follitropin receptor]

[FSH]

[FSHR; FSHR; LGR1; ODG1; FSHR1; FSHRO; LGR1; FSH-R]

FSHR_HUMAN

Rat

669

This assay has high sensitivity and excellent specificity for detection of Rat FSH. No significant cross-reactivity or interference between Rat FSH and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the crossreactivity detection between Rat FSH and all the analogues, therefore, cross reaction may still exist.

Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Samples: Serum, plasma and other biological fluids. Assay Type: Competitive ELISA, Coated with Antigen. Detection Range: 2.344-150mIU/ml. Sensitivity: 1.406mIU/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Rat FSH were tested 20 times on one plate, respectively. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Rat FSH were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<10%

Principle of the Assay This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with Rat FSH. During the reaction,Rat FSH in the sample or standard competes with a fixed amount of Rat FSH on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to Rat FSH. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Rat FSH in the samples is then determined by comparing the O.D. of the samples to the standard curve.

291575177

NP_852111.2

NM_181446.2

81,974 Da

Class A/1 (Rhodopsin-like Receptors) Pathway (1269545); FSH Signaling Pathway (672455); G Alpha (s) Signalling Events Pathway (1269575); GPCR Downstream Signaling Pathway (1269574); GPCR Ligand Binding Pathway (1269544); GPCRs, Class A Rhodopsin-like Pathway (198886); GPCRs, Other Pathway (198765); Hormone Ligand-binding Receptors Pathway (1269559); Neuroactive Ligand-receptor Interaction Pathway (83053); Neuroactive Ligand-receptor Interaction Pathway (462)

The protein encoded by this gene belongs to family 1 of G-protein coupled receptors. It is the receptor for follicle stimulating hormone and functions in gonad development. Mutations in this gene cause ovarian dysgenesis type 1, and also ovarian hyperstimulation syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010]

FSHR: Receptor for follicle-stimulating hormone. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. Defects in FSHR are a cause of ovarian dysgenesis type 1 (ODG1); also known as premature ovarian failure or gonadal dysgenesis XX type or XX gonadal dysgenesis (XXGD) or hereditary hypergonadotropic ovarian failure or hypergonadotropic ovarian dysgenesis with normal karyotype. ODG1 is an autosomal recessive disease characterized by primary amenorrhea, variable development of secondary sex characteristics, and high serum levels of follicle-stimulating hormone (FSH) and luteinizing hormone (LH). Defects in FSHR are a cause of ovarian hyperstimulation syndrome (OHSS). OHSS is a disorder which occurs either spontaneously or most often as an iatrogenic complication of ovarian stimulation treatments for in vitro fertilization. The clinical manifestations vary from abdominal distention and discomfort to potentially life-threatening, massive ovarian enlargement and capillary leak with fluid sequestration. Pathologic features of this syndrome include the presence of multiple serous and hemorrhagic follicular cysts lined by luteinized cells, a condition called hyperreactio luteinalis. Belongs to the G-protein coupled receptor 1 family. FSH/LSH/TSH subfamily. 3 isoforms of the human protein are produced by alternative splicing. Protein type: GPCR, family 1; Membrane protein, multi-pass; Receptor, GPCR; Membrane protein, integral. Chromosomal Location of Human Ortholog: 2p21-p16. Cellular Component: integral to membrane; integral to plasma membrane; plasma membrane. Molecular Function: follicle-stimulating hormone receptor activity; peptide receptor activity, G-protein coupled; protein binding. Biological Process: adenylate cyclase activation; female gamete generation; female gonad development; follicle-stimulating hormone signaling pathway; G-protein coupled receptor protein signaling pathway; G-protein signaling, adenylate cyclase activating pathway; gonad development; hormone-mediated signaling; male gonad development; positive regulation of phosphoinositide 3-kinase cascade; primary ovarian follicle growth; regulation of osteoclast differentiation; spermatogenesis. Disease: Ovarian Dysgenesis 1; Ovarian Hyperstimulation Syndrome; Twinning, Dizygotic

48-Strip-Wells

300 USD

96-Strip-Wells

415

5x96-Strip-Wells

1825

10x96-Strip-Wells

3425