Human Tryptase ELISA Kit | MyBioSource MBS764688 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Tryptase ELISA Kit

catalog :

MBS764688

quantity :

48-Strip-Wells

price :

300 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS764688

products type :

ELISA Kit

products full name :

Human Tryptase ELISA Kit

products short name :

[Tryptase]

products name syn :

[TPS]

other names :

[tryptase; Tryptase alpha/beta-1; tryptase alpha/beta-1; tryptase alpha/beta 1; Tryptase I; Tryptase alpha-1]

products gene name :

[TPS]

other gene names :

[TPSAB1; TPSAB1; TPS1; TPS2; TPSB1; TPS1; TPS2; TPSB1; Tryptase-1]

uniprot entry name :

TRYB1_HUMAN

reactivity :

Human

sequence length :

275

specificity :

This assay has high sensitivity and excellent specificity for detection of TPO. No significant cross-reactivity or interference between TPO and analogues was observed.

storage stability :

Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids. Assay Type: Sandwich. Detection Range: 31.25-2000pg/ml. Sensitivity: <18.75pg/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level TPO were tested 20 times on one plate, respectively. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level TPO were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%

products description :

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-TPO antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-TPO antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the TPO amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of TPO can be calculated.

ncbi gi num :

1182067

ncbi acc num :

AAA86934.1

ncbi mol weight :

29,533 Da

ncbi pathways :

Activation Of Matrix Metalloproteinases Pathway (1270258); Degradation Of The Extracellular Matrix Pathway (1270257); Extracellular Matrix Organization Pathway (1270244)

ncbi summary :

Tryptases comprise a family of trypsin-like serine proteases, the peptidase family S1. Tryptases are enzymatically active only as heparin-stabilized tetramers, and they are resistant to all known endogenous proteinase inhibitors. Several tryptase genes are clustered on chromosome 16p13.3. These genes are characterized by several distinct features. They have a highly conserved 3' UTR and contain tandem repeat sequences at the 5' flank and 3' UTR which are thought to play a role in regulation of the mRNA stability. These genes have an intron immediately upstream of the initiator Met codon, which separates the site of transcription initiation from protein coding sequence. This feature is characteristic of tryptases but is unusual in other genes. The alleles of this gene exhibit an unusual amount of sequence variation, such that the alleles were once thought to represent two separate genes, alpha and beta 1. Beta tryptases appear to be the main isoenzymes expressed in mast cells; whereas in basophils, alpha tryptases predominate. Tryptases have been implicated as mediators in the pathogenesis of asthma and other allergic and inflammatory disorders. [provided by RefSeq, Jul 2008]

uniprot summary :

TPSAB1: Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. Belongs to the peptidase S1 family. Tryptase subfamily. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted; Protease; EC 3.4.21.59; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 16p13.3. Cellular Component: extracellular matrix; extracellular region; extracellular space. Molecular Function: protein binding; serine-type endopeptidase activity; serine-type peptidase activity. Biological Process: defense response; extracellular matrix disassembly; extracellular matrix organization and biogenesis; proteolysis

size1 :

48-Strip-Wells

price1 :

300 USD

size2 :

96-Strip-Wells

price2 :

415

size3 :

5x96-Strip-Wells

price3 :

1825

size4 :

10x96-Strip-Wells

price4 :

3425

more info or order :

MyBioSource product webpage