This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
MyBioSource
product type :
ELISA/assay
product name :
Rat Interleukin 1 Beta ELISA Kit
catalog :
MBS764668
quantity :
48-Strip-Wells
price :
300 USD
product information
catalog number :
MBS764668
products type :
ELISA Kit
products full name :
Rat Interleukin 1 Beta ELISA Kit
products short name :
[Interleukin 1 Beta]
products name syn :
[IL-1b/IL-1beta/IL1B/IL1-BETA/IL1F2/catabolin ; Interleukin 1 Beta]
other names :
[interleukin 1 beta; interleukin 1 beta; interleukin 1 beta]
products gene name :
[IL-1beta]
products gene name syn :
[IL-1b]
other gene names :
[il1b; IL-1; IL-1B; il1b-A]
reactivity :
Rat
sequence length :
283
specificity :
This assay has high sensitivity and excellent specificity for detection of IL-1beta. No significant cross-reactivity or interference between IL-1beta and analogues was observed.
storage stability :
Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
image1 heading :
Typical Standard Curve/Testing Data
other info1 :
Samples: Serum, plasma, tissue homogenates and other biological fluids. Assay Type: Quantitative Sandwich. Detection Range: 31.25-2000pg/ml. Sensitivity: <18.75pg/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level IL-1 beta were tested 20 times on one plate, respectively. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level IL-1 beta were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
products description :
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- IL-1beta antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- IL-1beta antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IL-1beta amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of IL-1beta can be calculated.
ncbi gi num :
148226412
ncbi acc num :
NP_001079074.1
ncbi gb acc num :
NM_001085605.1
ncbi pathways :
AGE-RAGE Signaling Pathway In Diabetic Complications (1320296); AGE-RAGE Signaling Pathway In Diabetic Complications (1319775); Apoptosis Pathway (84655); Apoptosis Pathway (470); Cytokine-cytokine Receptor Interaction Pathway (119282); Cytokine-cytokine Receptor Interaction Pathway (460); Cytosolic DNA-sensing Pathway (125125); Cytosolic DNA-sensing Pathway (124833); Herpes Simplex Infection Pathway (377871); Herpes Simplex Infection Pathway (377865)
size1 :
48-Strip-Wells
price1 :
300 USD
size2 :
96-Strip-Wells
price2 :
415
size3 :
5x96-Strip-Wells
price3 :
1825
size4 :
10x96-Strip-Wells
price4 :
3425
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

"MyBioSource offers the best products at unbeatable prices."

Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.

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