This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
MyBioSource
product type :
ELISA/assay
product name :
Mouse Monocyte Chemotactic Protein 1 ELISA Kit
catalog :
MBS764665
quantity :
48-Strip-Wells
price :
300 USD
product information
catalog number :
MBS764665
products type :
ELISA Kit
products full name :
Mouse Monocyte Chemotactic Protein 1 ELISA Kit
products short name :
[Monocyte Chemotactic Protein 1]
products name syn :
[MCP-1/CCL2/GDCF-2/HC11/HSMCR30/MCAF/MCP1/SCYA2/SMC-CF]
other names :
[monocyte chemotactic protein-1, partial; Monocyte chemotactic protein-1; Monocyte chemotactic protein-1]
products gene name :
[MCP-1]
other gene names :
[MCP-1]
uniprot entry name :
A0A075IJR0_MUSPF
reactivity :
Mouse
sequence length :
44
specificity :
This assay has high sensitivity and excellent specificity for detection of MCP-1. No significant cross-reactivity or interference between MCP-1 and analogues was observed.
storage stability :
Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids. Assay Type: Sandwich. Detection Range: 0.156-10ng/ml. Sensitivity: <0.094ng/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level MCP-1 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level MCP-1 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
products description :
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-MCP-1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-MCP-1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the MCP-1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of MCP-1 can be calculated.
ncbi gi num :
663497593
ncbi acc num :
AIF30289.1
ncbi mol weight :
5,028 Da
size1 :
48-Strip-Wells
price1 :
300 USD
size2 :
96-Strip-Wells
price2 :
415
size3 :
5x96-Strip-Wells
price3 :
1825
size4 :
10x96-Strip-Wells
price4 :
3425
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

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