Mouse Formyl Peptide Receptor 2 ELISA Kit | MyBioSource MBS764510 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Formyl Peptide Receptor 2 ELISA Kit

catalog :

MBS764510

quantity :

48-Strip-Wells

price :

300 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS764510

products type :

ELISA Kit

products full name :

Mouse Formyl Peptide Receptor 2 ELISA Kit

products short name :

[Formyl Peptide Receptor 2]

products name syn :

[FPR2/RFP/Lipoxin A4 receptor/LXA4 receptor/HM63/FMLP-related receptor I/FMLP-R-I/Formyl peptide receptor-like 1/FPRH1/FPRL1/LXA4R]

other names :

[Formyl peptide receptor 2; N-formyl peptide receptor 2; N-formyl peptide receptor 2; formyl peptide receptor 2; FMLP-related receptor I; FMLP-R-I; Formyl peptide receptor-like 1; HM63; Lipoxin A4 receptor; LXA4 receptor; RFP]

products gene name :

[FPR2]

other gene names :

[FPR2; FPR2; ALXR; HM63; FMLPX; FPR2A; FPRH1; FPRH2; FPRL1; LXA4R; FMLP-R-II; FPRH1; FPRL1; LXA4R; FMLP-R-I; LXA4 receptor]

uniprot entry name :

FPR2_HUMAN

reactivity :

Mouse

sequence length :

351

specificity :

This assay has high sensitivity and excellent specificity for detection of FPR2 . No significant cross-reactivity or interference between FPR2 and analogues was observed. Note: Limited by current skills and knowledge, it is difficult for us to complete the crossreactivity detection between FPR2 and all the analogues, therefore, cross reaction may still exist.

storage stability :

Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, plasma, tissue homogenates and other biological fluids. Assay Type: Sandwich. Detection Range: 31.25-2000pg/ml. Sensitivity: <18.75pg/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level FPR2 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level FPR2 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%

products description :

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- FPR2 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- FPR2 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the FPR2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of FPR2 can be calculated.

ncbi gi num :

47940096

ncbi acc num :

AAH71722.1

ncbi mol weight :

38,964 Da

ncbi pathways :

Class A/1 (Rhodopsin-like Receptors) Pathway (1269545); Formyl Peptide Receptors Bind Formyl Peptides And Many Other Ligands Pathway (1269551); G Alpha (i) Signalling Events Pathway (1269576); G Alpha (q) Signalling Events Pathway (1269578); GPCR Downstream Signaling Pathway (1269574); GPCR Ligand Binding Pathway (1269544); GPCRs, Class A Rhodopsin-like Pathway (198886); Gastrin-CREB Signalling Pathway Via PKC And MAPK (1269592); Neuroactive Ligand-receptor Interaction Pathway (83053); Neuroactive Ligand-receptor Interaction Pathway (462)

uniprot summary :

FPR2: Low affinity receptor for N-formyl-methionyl peptides, which are powerful neutrophils chemotactic factors. Binding of FMLP to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. The activation of LXA4R could result in an anti-inflammatory outcome counteracting the actions of proinflammatory signals such as LTB4 (leukotriene B4). Belongs to the G-protein coupled receptor 1 family. Protein type: GPCR, family 1; Membrane protein, integral; Motility/polarity/chemotaxis; Receptor, GPCR; Membrane protein, multi-pass. Chromosomal Location of Human Ortholog: 19q13.3-q13.4. Cellular Component: integral to membrane; plasma membrane. Molecular Function: G-protein coupled receptor activity; N-formyl peptide receptor activity. Biological Process: cell adhesion; cell motility; chemotaxis; G-protein coupled receptor protein signaling pathway; inflammatory response

size1 :

48-Strip-Wells

price1 :

300 USD

size2 :

96-Strip-Wells

price2 :

415

size3 :

5x96-Strip-Wells

price3 :

1825

size4 :

10x96-Strip-Wells

price4 :

3425

more info or order :

MyBioSource product webpage