Rat Thrombopoietin ELISA Kit | MyBioSource MBS764453 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat Thrombopoietin ELISA Kit

catalog :

MBS764453

quantity :

48-Strip-Wells

price :

300 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS764453

products type :

ELISA Kit

products full name :

Rat Thrombopoietin ELISA Kit

products short name :

[Thrombopoietin]

products name syn :

[TPO (Thrombopoietin)/MGDF/MK-CSF/THPO]

other names :

[thrombopoietin isoform 1; Thrombopoietin; thrombopoietin; thrombopoietin; C-mpl ligand; ML; Megakaryocyte colony-stimulating factor; Megakaryocyte growth and development factor; MGDF; Myeloproliferative leukemia virus oncogene ligand]

products gene name :

[TPO]

other gene names :

[THPO; THPO; ML; TPO; MGDF; MKCSF; MPLLG; THCYT1; MGDF; ML; MGDF]

uniprot entry name :

TPO_HUMAN

reactivity :

Rat

sequence length :

353

specificity :

This assay has high sensitivity and excellent specificity for detection of TNX. No significant cross-reactivity or interference between TNX and analogues was observed.

storage stability :

Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids. Assay Type: Sandwich. Detection Range: 1.563-100ng/ml. Sensitivity: < 0.938ng/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level TNX were tested 20 times on one plate, respectively. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level TNX were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%

products description :

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-TNX antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-TNX antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the TNX amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of TNX can be calculated.

ncbi gi num :

586798208

ncbi acc num :

NP_001276927.1

ncbi gb acc num :

NM_001289998.1

ncbi mol weight :

33,604 Da

ncbi pathways :

Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Hematopoietic Cell Lineage Pathway (83078); Hematopoietic Cell Lineage Pathway (489); Hemostasis Pathway (1269340); Jak-STAT Signaling Pathway (83077); Jak-STAT Signaling Pathway (488); Platelet Aggregation (Plug Formation) Pathway (1269358); Platelet Activation, Signaling And Aggregation Pathway (1269350)

ncbi summary :

Megakaryocytopoiesis is the cellular development process that leads to platelet production. The main functional protein encoded by this gene is a humoral growth factor that is necessary for megakaryocyte proliferation and maturation, as well as for thrombopoiesis. This protein is the ligand for MLP/C_MPL, the product of myeloproliferative leukemia virus oncogene. Mutations in this gene are the cause of thrombocythemia 1. Alternative promoter usage and differential splicing result in multiple transcript variants differing in the 5' UTR and/or coding region. Multiple AUG codons upstream of the main open reading frame (ORF) have been identified, and these upstream AUGs inhibit translation of the main ORF at different extent. [provided by RefSeq, Feb 2014]

uniprot summary :

THPO: Lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage of megakaryocyte development. It may be the major physiological regulator of circulating platelets. Defects in THPO are the cause of thrombocythemia type 1 (THCYT1). A myeloproliferative disorder characterized by elevated platelet levels due to sustained proliferation of megakaryocytes, and frequently lead to thrombotic and haemorrhagic complications. Belongs to the EPO/TPO family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Cell cycle regulation; Cytokine; Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 3q27. Cellular Component: extracellular space. Molecular Function: cytokine activity; growth factor activity; hormone activity. Biological Process: blood coagulation; cell proliferation; multicellular organismal development; myeloid cell differentiation; platelet activation; positive regulation of megakaryocyte differentiation; positive regulation of protein amino acid phosphorylation; positive regulation of protein kinase B signaling cascade. Disease: Thrombocythemia 1

size1 :

48-Strip-Wells

price1 :

300 USD

size2 :

96-Strip-Wells

price2 :

415

size3 :

5x96-Strip-Wells

price3 :

1825

size4 :

10x96-Strip-Wells

price4 :

3425

more info or order :

MyBioSource product webpage