Human Transferrin ELISA Kit | MyBioSource MBS764132 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Transferrin ELISA Kit

catalog :

MBS764132

quantity :

48-Strip-Wells

price :

300 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS764132

products type :

ELISA Kit

products full name :

Human Transferrin ELISA Kit

products short name :

[Transferrin]

products name syn :

[TF (Transferrin)/PRO1557/PRO2086/TFQTL1/Siderophilin/Beta-1 metal-binding globulin/Beta-1 metal-binding globulin/serotransferrin/Serotransferrin]

other names :

[transferrin; Serotransferrin; serotransferrin; transferrin; Beta-1 metal-binding globulin; Siderophilin]

products gene name :

[TF]

other gene names :

[TF; TF; TFQTL1; PRO1557; PRO2086; HEL-S-71p; Transferrin]

uniprot entry name :

TRFE_HUMAN

reactivity :

Human

sequence length :

698

specificity :

This assay has high sensitivity and excellent specificity for detection of TET2. No significant cross-reactivity or interference between TET2 and analogues was observed.

storage stability :

Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids. Assay Type: Sandwich. Detection Range: 15.625-1000pg/ml. Sensitivity: <9.375pg/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level TET2 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level TET2 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%

products description :

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-TET2 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-TET2 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the TET2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of TET2 can be calculated.

ncbi gi num :

339453

ncbi acc num :

AAA61140.1

ncbi mol weight :

77,064 Da

ncbi pathways :

EPHB Forward Signaling Pathway (138047); HIF-1 Signaling Pathway (695200); HIF-1-alpha Transcription Factor Network Pathway (138045); Hemostasis Pathway (1269340); Iron Metabolism In Placenta Pathway (672461); Iron Uptake And Transport Pathway (1269948); Mineral Absorption Pathway (212237); Mineral Absorption Pathway (212220); Platelet Activation, Signaling And Aggregation Pathway (1269350); Platelet Degranulation Pathway (1269367)

ncbi summary :

This gene encodes a glycoprotein with an approximate molecular weight of 76.5 kDa. It is thought to have been created as a result of an ancient gene duplication event that led to generation of homologous C and N-terminal domains each of which binds one ion of ferric iron. The function of this protein is to transport iron from the intestine, reticuloendothelial system, and liver parenchymal cells to all proliferating cells in the body. This protein may also have a physiologic role as granulocyte/pollen-binding protein (GPBP) involved in the removal of certain organic matter and allergens from serum. [provided by RefSeq, Sep 2009]

uniprot summary :

TF: Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation. Defects in TF are the cause of atransferrinemia (ATRAF). Atransferrinemia is rare autosomal recessive disorder characterized by iron overload and hypochromic anemia. Belongs to the transferrin family. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 3q22.1. Cellular Component: apical plasma membrane; basal plasma membrane; cell surface; coated pit; cytoplasmic membrane-bound vesicle; early endosome; endocytic vesicle; endosome membrane; extracellular region; extracellular space; extrinsic to external side of plasma membrane; late endosome; perinuclear region of cytoplasm; recycling endosome; vesicle. Molecular Function: ferric iron binding; ferric iron transmembrane transporter activity; ferrous iron binding; protein binding. Biological Process: blood coagulation; cellular iron ion homeostasis; iron ion homeostasis; platelet activation; platelet degranulation; positive regulation of receptor-mediated endocytosis; regulation of protein stability; retinal homeostasis; transferrin transport; transmembrane transport. Disease: Atransferrinemia

size1 :

48-Strip-Wells

price1 :

300 USD

size2 :

96-Strip-Wells

price2 :

415

size3 :

5x96-Strip-Wells

price3 :

1825

size4 :

10x96-Strip-Wells

price4 :

3425

more info or order :

MyBioSource product webpage