Human Lysozyme ELISA Kit | MyBioSource MBS764112 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Lysozyme ELISA Kit

catalog :

MBS764112

quantity :

48-Strip-Wells

price :

300 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS764112

products type :

ELISA Kit

products full name :

Human Lysozyme ELISA Kit

products short name :

[Lysozyme]

products name syn :

[LZM/lysozyme/lysozyme (renal amyloidosis)1,4-beta-N-acetylmuramidase C/lysozyme C/LYZ/renal amyloidosis/1,4-beta-N-acetylmuramidase C]

other names :

[lysozyme (EC 3.2.1.17); Lysozyme C; lysozyme C; lysozyme; 1,4-beta-N-acetylmuramidase C]

products gene name :

[LZM]

other gene names :

[LYZ; LYZ; LZM; LYZF1; LZM]

uniprot entry name :

LYSC_HUMAN

reactivity :

Human

sequence length :

148

specificity :

This assay has high sensitivity and excellent specificity for detection of LZMg . No significant cross-reactivity or interference between LZMg and analogues was observed. Note: Limited by current skills and knowledge, it is difficult for us to complete the crossreactivity detection between LZMg and all the analogues, therefore, cross reaction may still exist.

storage stability :

Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, plasma, tissue homogenates and other biological fluids. Assay Type: Sandwich. Detection Range: 1.25-80ng/ml. Sensitivity: <0.75ng/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level LZMg were tested 20 times on one plate, respectively. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level LZMg were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%

products description :

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- LZMg antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- LZMg antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the LZMg amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of LZMg can be calculated.

ncbi gi num :

307142

ncbi acc num :

AAA59536.1

ncbi mol weight :

16,537 Da

ncbi pathways :

Amyloid Fiber Formation Pathway (1269169); C-MYB Transcription Factor Network Pathway (138073); Metabolism Of Proteins Pathway (1268677); Salivary Secretion Pathway (153376); Salivary Secretion Pathway (153352)

ncbi summary :

This gene encodes human lysozyme, whose natural substrate is the bacterial cell wall peptidoglycan (cleaving the beta[1-4]glycosidic linkages between N-acetylmuramic acid and N-acetylglucosamine). Lysozyme is one of the antimicrobial agents found in human milk, and is also present in spleen, lung, kidney, white blood cells, plasma, saliva, and tears. The protein has antibacterial activity against a number of bacterial species. Missense mutations in this gene have been identified in heritable renal amyloidosis. [provided by RefSeq, Oct 2014]

uniprot summary :

LYZ: Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte- macrophage system and enhance the activity of immunoagents. Defects in LYZ are a cause of amyloidosis type 8 (AMYL8); also known as systemic non-neuropathic amyloidosis or Ostertag-type amyloidosis. AMYL8 is a hereditary generalized amyloidosis due to deposition of apolipoprotein A1, fibrinogen and lysozyme amyloids. Viscera are particularly affected. There is no involvement of the nervous system. Clinical features include renal amyloidosis resulting in nephrotic syndrome, arterial hypertension, hepatosplenomegaly, cholestasis, petechial skin rash. Belongs to the glycosyl hydrolase 22 family. Protein type: Endoplasmic reticulum; Vesicle; Secreted; Secreted, signal peptide; Hydrolase; EC 3.2.1.17. Chromosomal Location of Human Ortholog: 12q15. Cellular Component: extracellular region; extracellular space. Molecular Function: identical protein binding; lysozyme activity. Biological Process: cellular protein metabolic process; cytolysis; defense response to bacterium; inflammatory response; retinal homeostasis. Disease: Amyloidosis, Familial Visceral

size1 :

48-Strip-Wells

price1 :

300 USD

size2 :

96-Strip-Wells

price2 :

415

size3 :

5x96-Strip-Wells

price3 :

1825

size4 :

10x96-Strip-Wells

price4 :

3425

more info or order :

MyBioSource product webpage