Human Interleukin 17 ELISA Kit | MyBioSource MBS764076 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Interleukin 17 ELISA Kit

catalog :

MBS764076

quantity :

48-Strip-Wells

price :

300 USD

product information

catalog number :

MBS764076

products type :

ELISA Kit

products full name :

Human Interleukin 17 ELISA Kit

products short name :

[Interleukin 17]

products name syn :

[IL-17 (Interleukin 17)/CTLA-8/IL17A/IL-17CTLA-8/IL17interleukin-17A/interleukin 17A]

other names :

[interleukin-17, partial; Interleukin-17A; interleukin-17A; interleukin 17A; Cytotoxic T-lymphocyte-associated antigen 8; CTLA-8]

products gene name :

[IL-17]

other gene names :

[IL17A; IL17A; IL17; CTLA8; IL-17; CTLA-8; IL-17A; CTLA8; IL17; IL-17; IL-17A; CTLA-8]

uniprot entry name :

IL17_HUMAN

reactivity :

Human

sequence length :

specificity :

This assay has high sensitivity and excellent specificity for detection of IL-12A. No significant cross-reactivity or interference between IL-12A and analogues was observed.

storage stability :

Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids. Assay Type: Sandwich. Detection Range: 15.625-1000pg/ml. Sensitivity: <9.375pg/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level IL-12A were tested 20 times on one plate, respectively. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level IL-12A were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%

products description :

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-IL-12A antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-IL-12A antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IL-12A amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of IL-12A can be calculated.

ncbi gi num :

55139064

ncbi acc num :

AAV41220.1

ncbi mol weight :

17,504 Da

ncbi pathways :

Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); IL17 Signaling Pathway (920968); IL23-mediated Signaling Events Pathway (138000); IL27-mediated Signaling Events Pathway (137929); Inflammatory Bowel Disease (IBD) Pathway (842771); Inflammatory Bowel Disease (IBD) Pathway (842797); Rheumatoid Arthritis Pathway (200309); Rheumatoid Arthritis Pathway (200269); TCR Signaling Pathway (198862)

ncbi summary :

The protein encoded by this gene is a proinflammatory cytokine produced by activated T cells. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis. [provided by RefSeq, Jul 2008]

uniprot summary :

IL17A: Induces stromal cells to produce proinflammatory and hematopoietic cytokines. Enhances the surface expression of ICAM1/intracellular adhesion molecule 1 in fibroblasts. Belongs to the IL-17 family. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 6p12. Cellular Component: cytoplasm; external side of plasma membrane; extracellular space. Molecular Function: cytokine activity; hematopoietin/interferon-class (D200-domain) cytokine receptor binding; protein binding. Biological Process: apoptosis; cell death; cell surface receptor linked signal transduction; cell-cell signaling; defense response to fungus; immune response; inflammatory response; positive regulation of interleukin-23 production; positive regulation of osteoclast differentiation; positive regulation of transcription from RNA polymerase II promoter; response to amino acid stimulus

size1 :

48-Strip-Wells

price1 :

300 USD

size2 :

96-Strip-Wells

price2 :

415

size3 :

5x96-Strip-Wells

price3 :

1825

size4 :

10x96-Strip-Wells

price4 :

3425