Human Vitamin D Binding Protein ELISA Kit | MyBioSource MBS763939 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Vitamin D Binding Protein ELISA Kit

catalog :

MBS763939

quantity :

48-Strip-Wells

price :

300 USD

product information

catalog number :

MBS763939

products type :

ELISA Kit

products full name :

Human Vitamin D Binding Protein ELISA Kit

products short name :

[Vitamin D Binding Protein]

products name syn :

[DBP/Vitamin D Binding Protein/DBP-MAF/Gc/Gc-MAF/VDBG/VDBP/DBP/GC/DBPVDBG/EC 6.3.1.5/Gc-globulin/gc-globulin/GRD3/Group-specific component/group-specific component (vitamin D binding protein)/hDBP/VDB/VDBP/vitamin D-binding alpha-globulin/vitamin D-binding protein/]

other names :

[vitamin D-binding protein isoform 3; Vitamin D-binding protein; vitamin D-binding protein; group-specific component (vitamin D binding protein); Gc protein-derived macrophage activating factor]

other gene names :

[GC; GC; DBP; GRD3; VDBG; VDBP; GcMAF; DBP/GC; Gc-MAF; HEL-S-51; DBP-maf]

uniprot entry name :

VTDB_HUMAN

reactivity :

Human

sequence length :

493

specificity :

This assay has high sensitivity and excellent specificity for detection of DBP. No significant cross-reactivity or interference between DBP and analogues was observed.

storage stability :

Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids. Assay Type: Sandwich. Detection Range: 15.625-1000ng/ml. Sensitivity: <9.375ng/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level DBP were tested 20 times on one plate, respectively. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level DBP were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%

products description :

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-DBP antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-DBP antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the DBP amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of DBP can be calculated.

ncbi gi num :

324021745

ncbi acc num :

NP_001191236.1

ncbi gb acc num :

NM_001204307.1

ncbi mol weight :

55,123 Da

ncbi pathways :

Metabolism Pathway (1269956); Metabolism Of Fat-soluble Vitamins Pathway (1339147); Metabolism Of Vitamins And Cofactors Pathway (1270144); Vitamin D (calciferol) Metabolism Pathway (1270052); Vitamin D Synthesis Pathway (198764)

ncbi summary :

The protein encoded by this gene belongs to the albumin gene family. It is a multifunctional protein found in plasma, ascitic fluid, cerebrospinal fluid and on the surface of many cell types. It binds to vitamin D and its plasma metabolites and transports them to target tissues. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Feb 2011]

uniprot summary :

GC: Multifunctional protein found in plasma, ascitic fluid, cerebrospinal fluid, and urine and on the surface of many cell types. In plasma, it carries the vitamin D sterols and prevents polymerization of actin by binding its monomers. DBP associates with membrane-bound immunoglobulin on the surface of B-lymphocytes and with IgG Fc receptor on the membranes of T-lymphocytes. Belongs to the ALB/AFP/VDB family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 4q12-q13. Cellular Component: axon; cytosol; extracellular region; extracellular space; lysosomal lumen; perinuclear region of cytoplasm. Molecular Function: actin binding; vitamin D binding; vitamin transporter activity. Biological Process: fat-soluble vitamin metabolic process; female pregnancy; lactation; response to estradiol stimulus; response to nutrient levels; vitamin D metabolic process; vitamin metabolic process; vitamin transport. Disease: Graves Disease, Susceptibility To, 1

size1 :

48-Strip-Wells

price1 :

300 USD

size2 :

96-Strip-Wells

price2 :

415

size3 :

5x96-Strip-Wells

price3 :

1825

size4 :

10x96-Strip-Wells

price4 :

3425