ELISA/assay

Mouse Vitamin K-dependent protein S ELISA Kit

MBS763916

48-Strip-Wells

300 USD

ELISA Kit

Mouse Vitamin K-dependent protein S ELISA Kit

[Vitamin K-dependent protein S]

[Pros1/EC 3.4.21/PROS/protein S (alpha)/protein Sa/PS21/PS22/PS23/PS24/PS25/PSA/vitamin K-dependent plasma protein S/vitamin K-dependent protein S]

[vitamin K-dependent protein S isoform 2 preproprotein; Vitamin K-dependent protein S; vitamin K-dependent protein S; protein S (alpha)]

[Pros1]

[PROS1; PROS1; PSA; PROS; PS21; PS22; PS23; PS24; PS25; THPH5; THPH6; PROS]

PROS_HUMAN

Mouse

676

This assay has high sensitivity and excellent specificity for detection of PROS1. No significant cross-reactivity or interference between PROS1 and analogues was observed.

Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids. Assay Type: Sandwich. Detection Range: 6.25-400ng/ml. Sensitivity: <3.75ng/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level PROS1 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level PROS1 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-PROS1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-PROS1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the PROS1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of PROS1 can be calculated.

192447438

NP_000304.2

NM_000313.3

75,123 Da

Cell Surface Interactions At The Vascular Wall Pathway (1269373); Common Pathway Of Fibrin Clot Formation (1269371); Complement And Coagulation Cascades Pathway (198880); Complement And Coagulation Cascades Pathway (83073); Complement And Coagulation Cascades Pathway (484); Complement Cascade Pathway (1269241); Formation Of Fibrin Clot (Clotting Cascade) Pathway (1269368); Gamma Carboxylation, Hypusine Formation And Arylsulfatase Activation Pathway (1268702); Gamma-carboxylation Of Protein Precursors Pathway (1268704); Gamma-carboxylation, Transport, And Amino-terminal Cleavage Of Proteins Pathway (1268703)

This gene encodes a vitamin K-dependent plasma protein that functions as a cofactor for the anticoagulant protease, activated protein C (APC) to inhibit blood coagulation. It is found in plasma in both a free, functionally active form and also in an inactive form complexed with C4b-binding protein. Mutations in this gene result in autosomal dominant hereditary thrombophilia. An inactive pseudogene of this locus is located at an adjacent region on chromosome 3. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar processing to generate mature protein. [provided by RefSeq, Oct 2015]

PROS1: Anticoagulant plasma protein; it is a cofactor to activated protein C in the degradation of coagulation factors Va and VIIIa. It helps to prevent coagulation and stimulating fibrinolysis. Defects in PROS1 are the cause of thrombophilia due to protein S deficiency, autosomal dominant (THPH5). A hemostatic disorder characterized by impaired regulation of blood coagulation and a tendency to recurrent venous thrombosis. However, many adults with heterozygous disease may be asymptomatic. Based on the plasma levels of total and free PROS1 antigen as well as the serine protease-activated protein C cofactor activity, three types of PROS1D have been described: type I, characterized by reduced total and free PROS1 antigen levels together with reduced anticoagulant activity; type III, in which only free PROS1 antigen and PROS1 activity levels are reduced; and the rare type II which is characterized by normal concentrations of both total and free PROS1 antigen, but low cofactor activity. Defects in PROS1 are the cause of thrombophilia due to protein S deficiency, autosomal recessive (THPH6). A very rare and severe hematologic disorder resulting in thrombosis and secondary hemorrhage usually beginning in early infancy. Some affected individuals develop neonatal purpura fulminans, multifocal thrombosis, or intracranial hemorrhage. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 3q11.2. Cellular Component: endoplasmic reticulum membrane; extracellular region; extracellular space; Golgi lumen; Golgi membrane; plasma membrane; protein complex. Molecular Function: calcium ion binding; endopeptidase inhibitor activity; protein complex binding. Biological Process: blood coagulation; cellular protein metabolic process; ER to Golgi vesicle-mediated transport; fibrinolysis; innate immune response; leukocyte migration; peptidyl-glutamic acid carboxylation; platelet activation; platelet degranulation; positive regulation of phagocytosis; post-translational protein modification; regulation of complement activation; response to lipopolysaccharide; signal peptide processing. Disease: Thrombophilia Due To Protein S Deficiency, Autosomal Dominant; Thrombophilia Due To Protein S Deficiency, Autosomal Recessive

48-Strip-Wells

300 USD

96-Strip-Wells

415

5x96-Strip-Wells

1825

10x96-Strip-Wells

3425