ELISA/assay

Human Kallikrein-2 ELISA Kit

MBS763900

48-Strip-Wells

300 USD

ELISA Kit

Human Kallikrein-2 ELISA Kit

[Kallikrein-2]

[KLK2 (Kallikrein-2)/Glandular kallikrein-1 (hGK-1)/Tissue kallikrein-2]

[kallikrein-2 isoform 1 preproprotein; Kallikrein-2; kallikrein-2; kallikrein related peptidase 2; Glandular kallikrein-1; hGK-1; Tissue kallikrein-2]

[KLK2]

[KLK2; KLK2; hK2; hGK-1; KLK2A2; hGK-1]

KLK2_HUMAN

Human

261

Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich ELISA, Double Antibody. Detection Range: 0.156-10ng/ml. Sensitivity: 0.094ng/ml

Principle of the Assay This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Human KLK2 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- Human KLK2 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human KLK2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human KLK2 can be calculated.

5031829

NP_005542.1

NM_005551.4

17,301 Da

Activated PKN1 Stimulates Transcription Of AR (androgen Receptor) Regulated Genes KLK2 And KLK3 Pathway (1269513); Activation Of Matrix Metalloproteinases Pathway (1270258); Coregulation Of Androgen Receptor Activity Pathway (138085); Degradation Of The Extracellular Matrix Pathway (1270257); Endocrine And Other Factor-regulated Calcium Reabsorption Pathway (213307); Endocrine And Other Factor-regulated Calcium Reabsorption Pathway (213276); Extracellular Matrix Organization Pathway (1270244); Metabolism Of Proteins Pathway (1268677); Prostate Cancer Pathway (755440); RHO GTPase Effectors Pathway (1269509)

This gene encodes a member of the grandular kallikrein protein family. Kallikreins are a subgroup of serine proteases that are clustered on chromosome 19. Members of this family are involved in a diverse array of biological functions. The protein encoded by this gene is a highly active trypsin-like serine protease that selectively cleaves at arginine residues. This protein is primarily expressed in prostatic tissue and is responsible for cleaving pro-prostate-specific antigen into its enzymatically active form. This gene is highly expressed in prostate tumor cells and may be a prognostic maker for prostate cancer risk. Alternate splicing results in both coding and non-coding transcript variants. [provided by RefSeq, Jan 2012]

KLK2: Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin. Belongs to the peptidase S1 family. Kallikrein subfamily. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Protease; EC 3.4.21.35. Chromosomal Location of Human Ortholog: 19q13.41. Cellular Component: extracellular region; intracellular. Molecular Function: serine-type endopeptidase activity. Biological Process: extracellular matrix disassembly; extracellular matrix organization and biogenesis; proteolysis; small GTPase mediated signal transduction

48-Strip-Wells

300 USD

96-Strip-Wells

415

5x96-Strip-Wells

1825

10x96-Strip-Wells

3425