catalog number :
MBS763696
products type :
ELISA Kit
products full name :
Mouse Ovalbumin Specific IgG ELISA Kit
products short name :
[Ovalbumin Specific IgG]
products name syn :
[OVA sIgG]
products gene name :
[OVA sIgG]
specificity :
This assay has high sensitivity and excellent specificity for detection of OVA sIgE. No significant cross-reactivity or interference between OVA sIgE and analogues was observed.
storage stability :
Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids. Detection Range: 1.563-100ng/ml. Sensitivity: <0.938ng/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level OVA sIgE were tested 20 times on one plate, respectively. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level OVA sIgE were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
products description :
Principle of the Assay: This kit was based on indirect enzyme-linked immune-sorbent assay technology. OVA was precoated onto 96-well plates. And the HRP conjugated anti-Mouse IgE antibody was used as detection antibodies. The standards, test samples and HRP conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the OVA sIgE amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of OVA sIgE can be calculated.
size4 :
10x96-Strip-Wells