Human Secreted frizzled-related protein 1 ELISA Kit | MyBioSource MBS763348 product information

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product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Secreted frizzled-related protein 1 ELISA Kit

catalog :

MBS763348

quantity :

48-Strip-Wells

price :

300 USD

product information

catalog number :

MBS763348

products type :

ELISA Kit

products full name :

Human Secreted frizzled-related protein 1 ELISA Kit

products short name :

[Secreted frizzled-related protein 1]

products name syn :

[SFRP1/Secreted frizzled-related protein 1/Secreted apoptosis-related protein 2/FRP-1/sFRP-1/FRP/FRP1/FrzA/FRP-1/SARP2]

other names :

[secreted frizzled-related protein 1; Secreted frizzled-related protein 1; secreted frizzled-related protein 1; secreted frizzled-related protein 1]

products gene name :

[SFRP1]

other gene names :

[Sfrp1; Sfrp1; sFRP-1; AW011917; AW107218; AW742929; 2210415K03Rik; sFRP-1]

uniprot entry name :

SFRP1_MOUSE

reactivity :

Human

sequence length :

314

specificity :

This assay has high sensitivity and excellent specificity for detection of SFRP1. No significant cross-reactivity or interference between SFRP1 and analogues was observed.

storage stability :

Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

image1 heading :

Typical Standard Curve/Testing Data

other info1 :

Samples: Serum, plasma, tissue homogenates and other biological fluids. Assay Type: Quantitative Sandwich. Detection Range: 0.156-10ng/ml. Sensitivity: <0.094ng/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level SFRP1 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level SFRP1 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%

products description :

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- SFRP1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- SFRP1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the SFRP1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of SFRP1 can be calculated.

ncbi gi num :

65301472

ncbi acc num :

NP_038862.2

ncbi gb acc num :

NM_013834.3

ncbi mol weight :

35,413 Da

ncbi pathways :

Wnt Signaling Pathway NetPath (198351); Wnt Signaling Pathway (83258); Wnt Signaling Pathway (471)

uniprot summary :

SFRP1: Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP1 decreases intracellular beta-catenin levels. Has antiproliferative effects on vascular cells, in vitro and in vivo, and can induce, in vivo, an angiogenic response. In vascular cell cycle, delays the G1 phase and entry into the S phase. In kidney development, inhibits tubule formation and bud growth in metanephroi. Inhibits WNT1/WNT4-mediated TCF-dependent transcription. Interacts with WNT1, WNT2 and FRZD6. Interacts with WNT4 and WNT8. Down-regulated in colorectal and breast tumors. Up- regulated in uterine leiomyomas under high estrogenic conditions. Expression, in leiomyomal cells, also increased both under hypoxic and serum deprivation conditions. Widely expressed. Absent from lung, liver and peripheral blood leukocytes. Highest levels in heart and fetal kidney. Also expressed in testis, ovary, fetal brain and lung, leiomyomal cells, myometrial cells and vascular smooth muscle cells. Expressed in foreskin fibroblasts and in keratinocytes. Belongs to the secreted frizzled-related protein (sFRP) family. Protein type: Secreted; Secreted, signal peptide. Cellular Component: cell surface; cytosol; extracellular matrix; extracellular region; extracellular space; integral to membrane; intracellular; plasma membrane; proteinaceous extracellular matrix. Molecular Function: cysteine-type endopeptidase activity; drug binding; frizzled binding; G-protein coupled receptor activity; heparin binding; identical protein binding; protein binding; Wnt receptor activity; Wnt-protein binding. Biological Process: actin cytoskeleton organization and biogenesis; anterior/posterior pattern formation; cell differentiation; development of primary male sexual characteristics; digestive tract morphogenesis; dorsal/ventral axis specification; female gonad development; hemopoiesis; hemopoietic progenitor cell differentiation; male gonad development; multicellular organismal development; negative regulation of apoptosis; negative regulation of B cell differentiation; negative regulation of BMP signaling pathway; negative regulation of bone remodeling; negative regulation of cell growth; negative regulation of cell migration; negative regulation of cell proliferation; negative regulation of epithelial cell proliferation; negative regulation of fibroblast proliferation; negative regulation of insulin secretion; negative regulation of JNK activity; negative regulation of ossification; negative regulation of osteoblast differentiation; negative regulation of osteoblast proliferation; negative regulation of osteoclast differentiation; negative regulation of peptidyl-tyrosine phosphorylation; negative regulation of transcription, DNA-dependent; negative regulation of Wnt receptor signaling pathway; neural crest cell fate commitment; neural tube closure; neural tube development; positive regulation of apoptosis; positive regulation of cell growth; positive regulation of cell proliferation; positive regulation of epithelial cell proliferation; positive regulation of fat cell differentiation; positive regulation of smoothened signaling pathway; positive regulation of transcription, DNA-dependent; positive regulation of Wnt receptor signaling pathway; proteolysis; regulation of ossification; response to drug; response to organic cyclic substance; somatic stem cell maintenance; somitogenesis; Wnt receptor signaling pathway; Wnt receptor signaling pathway through beta-catenin; Wnt receptor signaling pathway, planar cell polarity pathway

size1 :

48-Strip-Wells

price1 :

300 USD

size2 :

96-Strip-Wells

price2 :

415

size3 :

5x96-Strip-Wells

price3 :

1825

size4 :

10x96-Strip-Wells

price4 :

3425