ELISA/assay

Rat Tumor Necrosis Factor Alpha ELISA Kit

MBS763321

48-Strip-Wells

300 USD

ELISA Kit

Rat Tumor Necrosis Factor Alpha ELISA Kit

[Tumor Necrosis Factor Alpha]

[TNFalpha (Tumor Necrosis Factor Alpha)/TNF-alpha/DIF/TNF-alpha/TNFA/TNFSF2; Tumor Necrosis Factor Alpha]

[tumor necrosis factor alpha; tumor necrosis factor alpha]

[TNF-alpha]

[TNF-a]

[tnfa]

Rat

250

This assay has high sensitivity and excellent specificity for detection of TNF-alpha. No significant cross-reactivity or interference between TNF-alpha and analogues was observed.

Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich ELISA, Double Antibody. Detection Range: 15.625-1000pg/ml. Sensitivity: 9.375pg/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level TNF- alpha were tested 20 times on one plate, respectively. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level TNF-alpha were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- TNF-alpha antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- TNF-alpha antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the TNF-alpha amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of TNF-alpha can be calculated.

83921651

NP_001033074.1

NM_001037985.1

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48-Strip-Wells

300 USD

96-Strip-Wells

415

5x96-Strip-Wells

1825

10x96-Strip-Wells

3425