Human Reticulon 4 ELISA Kit | MyBioSource MBS763171 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Reticulon 4 ELISA Kit

catalog :

MBS763171

quantity :

48-Strip-Wells

price :

300 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS763171

products type :

ELISA Kit

products full name :

Human Reticulon 4 ELISA Kit

products short name :

[Reticulon 4]

products name syn :

[RTN4/Reticulon-5/RTN-x/Neuroendocrine-specific protein C homolog/Neuroendocrine-specific protein (NSP)/Foocen/Neurite outgrowth inhibitor (Nogo protein)/]

other names :

[Reticulon 4; Reticulon-4; reticulon-4; reticulon 4; Foocen; Neurite outgrowth inhibitor; Nogo protein; Neuroendocrine-specific protein; NSP; Neuroendocrine-specific protein C homolog; RTN-x; Reticulon-5]

products gene name :

[RTN4]

other gene names :

[RTN4; RTN4; ASY; NSP; NOGO; NOGOC; RTN-X; NOGO-A; NSP-CL; Nogo-B; Nogo-C; RTN4-A; RTN4-C; RTN4-B1; RTN4-B2; NI220/250; Nbla00271; Nbla10545; KIAA0886; NOGO; Nogo protein; NSP]

uniprot entry name :

RTN4_HUMAN

reactivity :

Human

sequence length :

1192

specificity :

This assay has high sensitivity and excellent specificity for detection of RRM2B. No significant cross-reactivity or interference between RRM2B and analogues was observed.

storage stability :

Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids. Assay Type: Sandwich. Detection Range: 0.156-10ng/ml. Sensitivity: <0.094ng/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level RRM2B were tested 20 times on one plate, respectively. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level RRM2B were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%

products description :

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-RRM2B antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-RRM2B antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the RRM2B amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of RRM2B can be calculated.

ncbi gi num :

156914657

ncbi acc num :

AAI52556.1

ncbi mol weight :

108,450 Da

ncbi pathways :

Axonal Growth Inhibition (RHOA Activation) Pathway (1269458); Signal Transduction Pathway (1269379); Signalling By NGF Pathway (1269443); Spinal Cord Injury Pathway (739007); P75 NTR Receptor-mediated Signalling Pathway (1269445); P75(NTR)-mediated Signaling Pathway (138019); P75NTR Regulates Axonogenesis Pathway (1269456)

ncbi summary :

This gene belongs to the family of reticulon encoding genes. Reticulons are associated with the endoplasmic reticulum, and are involved in neuroendocrine secretion or in membrane trafficking in neuroendocrine cells. The product of this gene is a potent neurite outgrowth inhibitor which may also help block the regeneration of the central nervous system in higher vertebrates. Alternatively spliced transcript variants derived both from differential splicing and differential promoter usage and encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

uniprot summary :

Nogo: Developmental neurite growth regulatory factor with a role as a negative regulator of axon-axon adhesion and growth, and as a facilitator of neurite branching. Regulates neurite fasciculation, branching and extension in the developing nervous system. Involved in down-regulation of growth, stabilization of wiring and restriction of plasticity in the adult CNS. Regulates the radial migration of cortical neurons via an RTN4R-LINGO1 containing receptor complex. Isoform 2 reduces the anti-apoptotic activity of Bcl-xl and Bcl-2. This is likely consecutive to their change in subcellular location, from the mitochondria to the endoplasmic reticulum, after binding and sequestration. Isoform 2 and isoform 3 inhibit BACE1 activity and amyloid precursor protein processing. 6 isoforms of the human protein are produced by alternative splicing. Protein type: Endoplasmic reticulum; Membrane protein, integral; Inhibitor; Membrane protein, multi-pass; Motility/polarity/chemotaxis; Cell development/differentiation. Chromosomal Location of Human Ortholog: 2p16.3. Cellular Component: endoplasmic reticulum; integral to endoplasmic reticulum membrane; intracellular; nuclear envelope; plasma membrane. Molecular Function: protein binding. Biological Process: apoptosis; axonal fasciculation; axonogenesis; cerebral cortex radial glia guided migration; negative regulation of axon extension; negative regulation of axonogenesis; negative regulation of cell growth; nerve growth factor receptor signaling pathway; regulation of apoptosis; regulation of axonogenesis; regulation of cell migration

size1 :

48-Strip-Wells

price1 :

300 USD

size2 :

96-Strip-Wells

price2 :

415

size3 :

5x96-Strip-Wells

price3 :

1825

size4 :

10x96-Strip-Wells

price4 :

3425

more info or order :

MyBioSource product webpage