ELISA/assay

Human Dickkopf-related protein 1 ELISA Kit

MBS763163

48-Strip-Wells

300 USD

ELISA Kit

Human Dickkopf-related protein 1 ELISA Kit

[Dickkopf-related protein 1]

[DKK1/dickkopf (Xenopus laevis) homolog 1/dickkopf homolog 1 (Xenopus laevis)/dickkopf related protein-1/Dickkopf-1/dickkopf-related protein 1/Dkk-1/DKK-1/SKdickkopf-1 like]

[dickkopf-related protein 1; Dickkopf-related protein 1; dickkopf-related protein 1; dickkopf WNT signaling pathway inhibitor 1; SK]

[DKK-1]

[DKK1; DKK1; SK; DKK-1; Dickkopf-1; Dkk-1; hDkk-1]

DKK1_HUMAN

Human

266

This assay has high sensitivity and excellent specificity for detection of dectin 1. No significant cross-reactivity or interference between dectin 1 and analogues was observed.

Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids. Assay Type: Sandwich. Detection Range: 31.25-2000pg/ml. Sensitivity: <18.75pg/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level dectin 1 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level dectin 1 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-dectin 1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-dectin 1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the dectin 1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of dectin 1 can be calculated.

7110719

NP_036374.1

NM_012242.2

28,672 Da

Cardiac Progenitor Differentiation Pathway (712094); Direct P53 Effectors Pathway (137939); Disease Pathway (1268854); Diseases Of Signal Transduction Pathway (1268855); Misspliced LRP5 Mutants Have Enhanced Beta-catenin-dependent Signaling Pathway (1268923); Negative Regulation Of TCF-dependent Signaling By WNT Ligand Antagonists Pathway (1269608); Presenilin Action In Notch And Wnt Signaling Pathway (137914); Regulation Of Wnt-mediated Beta Catenin Signaling And Target Gene Transcription Pathway (169352); Signal Transduction Pathway (1269379); Signaling By WNT In Cancer Pathway (1268906)

This gene encodes a protein that is a member of the dickkopf family. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the WNT signaling pathway. Elevated levels of DKK1 in bone marrow plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma. [provided by RefSeq, Jul 2008]

DKK1: Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero- posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease. Interacts with LRP6. Interacts (via the C-termianl Cys- rich domain) with LRP5 (via beta-propeller regions 3 and 4); the interaction, enhanced by MESD and or KREMEN, antagonizes Wnt- mediated signaling. Placenta. Belongs to the dickkopf family. Protein type: Inhibitor; Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 10q11.2. Cellular Component: early endosome membrane; extracellular region; extracellular space; plasma membrane. Molecular Function: growth factor activity; low-density lipoprotein receptor binding; protein binding; receptor antagonist activity; signal transducer activity. Biological Process: cellular morphogenesis during differentiation; embryonic limb morphogenesis; endoderm formation; forebrain development; hair follicle development; mesoderm formation; negative regulation of BMP signaling pathway; negative regulation of mesodermal cell fate specification; negative regulation of peptidyl-serine phosphorylation; negative regulation of protein binding; negative regulation of skeletal muscle development; negative regulation of synaptogenesis; negative regulation of transcription from RNA polymerase II promoter; negative regulation of Wnt receptor signaling pathway; regulation of endodermal cell fate specification; regulation of receptor internalization; response to retinoic acid

48-Strip-Wells

300 USD

96-Strip-Wells

415

5x96-Strip-Wells

1825

10x96-Strip-Wells

3425