ELISA/assay

Mouse Trefoil Factor 2 ELISA Kit

MBS763147

48-Strip-Wells

300 USD

ELISA Kit

Mouse Trefoil Factor 2 ELISA Kit

[Trefoil Factor 2]

[TFF2/SML1/SP/Spasmolysin/Spasmolytic Polypeptide/SML1trefoil factor 2; SML1; human spasmolytic polypeptide (SP)10spasmolytic protein 1/SP/spasmolysin/Spasmolysin/Spasmolytic polypeptide/trefoil factor 2]

[trefoil factor 2; Trefoil factor 2; trefoil factor 2; trefoil factor 2; Spasmolysin; Spasmolytic polypeptide; SP]

[TFF2]

[TFF2; TFF2; SP; SML1; SML1; SP]

TFF2_HUMAN

Mouse

129

This assay has high sensitivity and excellent specificity for detection of TFF2 . No significant cross-reactivity or interference between TFF2 and analogues was observed.

Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, plasma, tissue homogenates and other biological fluids. Assay Type: Sandwich. Detection Range: 62.5-4000pg/ml. Sensitivity: <37.5pg/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level TFF2 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level TFF2 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- TFF2 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- TFF2 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the TFF2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of TFF2 can be calculated.

4885629

NP_005414.1

NM_005423.4

14,284 Da

Members of the trefoil family are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. They are stable secretory proteins expressed in gastrointestinal mucosa. Their functions are not defined, but they may protect the mucosa from insults, stabilize the mucus layer and affect healing of the epithelium. The encoded protein inhibits gastric acid secretion. This gene and two other related trefoil family member genes are found in a cluster on chromosome 21. [provided by RefSeq, Jul 2008]

TFF2: Inhibits gastrointestinal motility and gastric acid secretion. Could function as a structural component of gastric mucus, possibly by stabilizing glycoproteins in the mucus gel through interactions with carbohydrate side chains. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 21q22.3. Cellular Component: extracellular space; intracellular. Molecular Function: CXCR4 chemokine receptor binding; protein binding. Biological Process: calcium-mediated signaling; digestion; negative regulation of inflammatory response; negative regulation of macrophage activation; positive regulation of cell proliferation; regulation of cell migration

48-Strip-Wells

300 USD

96-Strip-Wells

415

5x96-Strip-Wells

1825

10x96-Strip-Wells

3425