ELISA/assay

Mouse Toll-like receptor 1 ELISA Kit

MBS763099

48-Strip-Wells

300 USD

ELISA Kit

Mouse Toll-like receptor 1 ELISA Kit

[Toll-like receptor 1]

[TLR1/CD281/CD281/CD281 antigen/DKFZp547I0610/DKFZp564I0682/KIAA0012MGC126311/MGC104956/MGC126312/rsc786/TIL/TIL. LPRS5/Toll/interleukin-1 receptor-like protein/toll-like receptor 1]

[toll-like receptor 1; Toll-like receptor 1; toll-like receptor 1; toll like receptor 1; Toll/interleukin-1 receptor-like protein; TIL; CD_antigen: CD281]

[TLR1]

[TLR1; TLR1; TIL; CD281; rsc786; TIL. LPRS5; KIAA0012; TIL]

TLR1_HUMAN

Mouse

786

This assay has high sensitivity and excellent specificity for detection of TIMP-1. No significant cross-reactivity or interference between TIMP-1 and analogues was observed.

Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids. Assay Type: Sandwich. Detection Range: 4.69-300pg/ml. Sensitivity: <2.813pg/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level TIMP-1 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level TIMP-1 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-TIMP-1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-TIMP-1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the TIMP-1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of TIMP-1 can be calculated.

41350337

NP_003254.2

NM_003263.3

90,291 Da

Activated TLR4 Signalling Pathway (1269236); Beta Defensins Pathway (1269267); Defensins Pathway (1269265); Disease Pathway (1268854); Diseases Associated With The TLR Signaling Cascade Pathway (1269157); Diseases Of Immune System Pathway (1269156); IRAK4 Deficiency (TLR2/4) Pathway (1269158); Immune System Pathway (1269170); Innate Immune System Pathway (1269203); MyD88 Deficiency (TLR2/4) Pathway (1269160)

The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is ubiquitously expressed, and at higher levels than other TLR genes. Different length transcripts presumably resulting from use of alternative polyadenylation site, and/or from alternative splicing, have been noted for this gene. [provided by RefSeq, Jul 2008]

TLR1: Participates in the innate immune response to microbial agents. Cooperates with TLR2 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Binds MYD88 (via TIR domain). Interacts with CNPY3. Interacts (via extracellular domain) with TLR2. Ligand binding induces the formation of a heterodimer with TLR2. Ubiquitous. Highly expressed in spleen, ovary, peripheral blood leukocytes, thymus and small intestine. Belongs to the Toll-like receptor family. Protein type: Membrane protein, integral. Chromosomal Location of Human Ortholog: 4p14. Cellular Component: Golgi apparatus; integral to plasma membrane; lipid raft; membrane; phagocytic vesicle membrane; plasma membrane. Molecular Function: protein binding; protein heterodimerization activity; receptor activity; transmembrane receptor activity. Biological Process: detection of triacylated bacterial lipoprotein; immune response; inflammatory response; innate immune response; macrophage activation; MyD88-dependent toll-like receptor signaling pathway; positive regulation of interleukin-6 biosynthetic process; positive regulation of tumor necrosis factor biosynthetic process; regulation of cytokine secretion; signal transduction; toll-like receptor 1 signaling pathway; toll-like receptor 2 signaling pathway; toll-like receptor 4 signaling pathway; toll-like receptor signaling pathway. Disease: Leprosy, Susceptibility To, 5

48-Strip-Wells

300 USD

96-Strip-Wells

415

5x96-Strip-Wells

1825

10x96-Strip-Wells

3425