ELISA/assay

Human Aquaporin-5 ELISA Kit

MBS763015

48-Strip-Wells

300 USD

ELISA Kit

Human Aquaporin-5 ELISA Kit

[Aquaporin-5]

[AQP5/AQP-5/Aquaporin 5]

[aquaporin-5; Aquaporin-5; aquaporin-5; aquaporin 5]

[AQP5]

[AQP5; AQP5; PPKB; AQP-5; AQP-5]

AQP5_HUMAN

Human

265

This assay has high sensitivity and excellent specificity for detection of AQP-3. No significant cross-reactivity or interference between AQP-3 and analogues was observed.

Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Tissue Homogenates And Other Biological Fluids. Assay Type: Sandwich. Detection Range: 0.313-20ng/ml. Sensitivity: <0.188ng/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level AQP-3 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level AQP-3 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-AQP-3 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-AQP-3 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the AQP-3 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of AQP-3 can be calculated.

4502183

NP_001642.1

NM_001651.3

28,292 Da

Aquaporin-mediated Transport Pathway (1269944); Passive Transport By Aquaporins Pathway (1269945); Salivary Secretion Pathway (153376); Salivary Secretion Pathway (153352); Stabilization And Expansion Of The E-cadherin Adherens Junction Pathway (137918); Transmembrane Transport Of Small Molecules Pathway (1269903)

Aquaporin 5 (AQP5) is a water channel protein. Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein (MIP or AQP0). Aquaporin 5 plays a role in the generation of saliva, tears and pulmonary secretions. AQP0, AQP2, AQP5, and AQP6 are closely related and all map to 12q13. [provided by RefSeq, Jul 2008]

AQP5: Forms a water-specific channel. Implicated in the generation of saliva, tears, and pulmonary secretions. Belongs to the MIP/aquaporin (TC 1.A.8) family. Protein type: Membrane protein, multi-pass; Channel, misc.; Transporter; Membrane protein, integral; Transporter, aquaporin family. Chromosomal Location of Human Ortholog: 12q13. Cellular Component: apical plasma membrane; basal plasma membrane; endoplasmic reticulum; integral to plasma membrane; microvillus; plasma membrane. Molecular Function: glycerol channel activity; protein binding; water channel activity. Biological Process: camera-type eye morphogenesis; carbon dioxide transport; excretion; glycerol transport; odontogenesis; pancreatic juice secretion; saliva secretion; transmembrane transport; water transport

48-Strip-Wells

300 USD

96-Strip-Wells

415

5x96-Strip-Wells

1825

10x96-Strip-Wells

3425