Mouse Insulin-like growth factor-binding protein 7 ELISA Kit | MyBioSource MBS762872 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Insulin-like growth factor-binding protein 7 ELISA Kit

catalog :

MBS762872

quantity :

48-Strip-Wells

price :

300 USD

product information

catalog number :

MBS762872

products type :

ELISA Kit

products full name :

Mouse Insulin-like growth factor-binding protein 7 ELISA Kit

products short name :

[Insulin-like growth factor-binding protein 7]

products name syn :

[IGFBP-7/AGM/FSTL2/IBP-7/IGFBP7/IGFBP-7v/IGFBPRP1/MAC25/PSF/RAMSVPS/TAF]

other names :

[insulin-like growth factor-binding protein 7 isoform 1; Insulin-like growth factor-binding protein 7; insulin-like growth factor-binding protein 7; insulin like growth factor binding protein 7; IGFBP-rP1; MAC25 protein; PGI2-stimulating factor; Prostacyclin-stimulating factor; Tumor-derived adhesion factor; TAF]

products gene name :

[IGFBP-7]

other gene names :

[IGFBP7; IGFBP7; AGM; PSF; TAF; FSTL2; IBP-7; MAC25; IGFBP-7; RAMSVPS; IGFBP-7v; IGFBPRP1; MAC25; PSF; IBP-7; IGF-binding protein 7; IGFBP-7; TAF]

uniprot entry name :

IBP7_HUMAN

reactivity :

Mouse

sequence length :

282

specificity :

This assay has high sensitivity and excellent specificity for detection of IGFBP-7. No significant cross-reactivity or interference between IGFBP-7 and analogues was observed.

storage stability :

Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich ELISA, Double Antibody. Detection Range: 78-5000pg/ml. Sensitivity: 46.9pg/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level IGFBP-7 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level IGFBP-7 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%

products description :

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- IGFBP-7 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- IGFBP-7 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IGFBP-7 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of IGFBP-7 can be calculated.

ncbi gi num :

4504619

ncbi acc num :

NP_001544.1

ncbi gb acc num :

NM_001553.2

ncbi mol weight :

28,860 Da

ncbi pathways :

Cellular Senescence Pathway (1270426); Cellular Responses To Stress Pathway (1270414); Senescence And Autophagy Pathway (198780); Senescence-Associated Secretory Phenotype (SASP) Pathway (1270431)

ncbi summary :

This gene encodes a member of the insulin-like growth factor (IGF)-binding protein (IGFBP) family. IGFBPs bind IGFs with high affinity, and regulate IGF availability in body fluids and tissues and modulate IGF binding to its receptors. This protein binds IGF-I and IGF-II with relatively low affinity, and belongs to a subfamily of low-affinity IGFBPs. It also stimulates prostacyclin production and cell adhesion. Alternatively spliced transcript variants encoding different isoforms have been described for this gene, and one variant has been associated with retinal arterial macroaneurysm (PMID:21835307). [provided by RefSeq, Dec 2011]

uniprot summary :

IGFBP7: Binds IGF-I and IGF-II with a relatively low affinity. Stimulates prostacyclin (PGI2) production. Stimulates cell adhesion. Defects in IGFBP7 are the cause of retinal arterial macroaneurysm with supravalvular pulmonic stenosis (RAMSVPS). RAMSVPS is an autosomal recessive condition characterized by the bilateral appearance of 'beading' along the major retinal arterial trunks, with the subsequent formation of macroaneurysms. Affected individuals also have supravalvular pulmonic stenosis, often requiring surgical correction. Protein type: Cell cycle regulation; Cell adhesion; Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 4q12. Cellular Component: extracellular matrix; extracellular region; extracellular space. Molecular Function: insulin-like growth factor binding; protein binding. Biological Process: cell adhesion; inner ear development; negative regulation of cell proliferation; regulation of cell growth. Disease: Retinal Arterial Macroaneurysm With Supravalvular Pulmonic Stenosis

size1 :

48-Strip-Wells

price1 :

300 USD

size2 :

96-Strip-Wells

price2 :

415

size3 :

5x96-Strip-Wells

price3 :

1825

size4 :

10x96-Strip-Wells

price4 :

3425