Human Complement Component 3a ELISA Kit | MyBioSource MBS762839 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Complement Component 3a ELISA Kit

catalog :

MBS762839

quantity :

48-Strip-Wells

price :

300 USD

product information

catalog number :

MBS762839

products type :

ELISA Kit

products full name :

Human Complement Component 3a ELISA Kit

products short name :

[Complement Component 3a]

products name syn :

[C3a/C3a-desArg/ASP/Anaphylatoxin/C3]

other names :

[complement component 3a receptor 1; C3a anaphylatoxin chemotactic receptor; C3a anaphylatoxin chemotactic receptor; complement component 3a receptor 1]

products gene name :

[C3a]

other gene names :

[C3AR1; C3AR1; AZ3B; C3AR; HNFAG09; AZ3B; C3R1; HNFAG09; C3AR; C3a-R]

uniprot entry name :

C3AR_HUMAN

reactivity :

Human

sequence length :

482

specificity :

This assay has high sensitivity and excellent specificity for detection of C3a. No significant cross-reactivity or interference between C3a and analogues was observed.

storage stability :

Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: serum, plasma, tissue homogenates and other biological fluids. Assay Type: Quantitative Sandwich. Detection Range: 0.313-20ng/ml. Sensitivity: <0.188ng/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level C3a were tested 20 times on one plate, respectively. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level C3a were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%

products description :

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- C3a antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- C3a antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the C3a amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of C3a can be calculated.

ncbi gi num :

30385606

ncbi acc num :

AAP23198.1

ncbi mol weight :

53,864 Da

ncbi pathways :

Class A/1 (Rhodopsin-like Receptors) Pathway (1269545); Complement And Coagulation Cascades Pathway (198880); Complement And Coagulation Cascades Pathway (83073); Complement And Coagulation Cascades Pathway (484); G Alpha (i) Signalling Events Pathway (1269576); GPCR Downstream Signaling Pathway (1269574); GPCR Ligand Binding Pathway (1269544); GPCRs, Class A Rhodopsin-like Pathway (198886); Neuroactive Ligand-receptor Interaction Pathway (83053); Neuroactive Ligand-receptor Interaction Pathway (462)

uniprot summary :

C3aR: Receptor for the chemotactic and inflammatory peptide anaphylatoxin C3a. This receptor stimulates chemotaxis, granule enzyme release and superoxide anion production. Belongs to the G-protein coupled receptor 1 family. Protein type: Membrane protein, multi-pass; Membrane protein, integral; Receptor, GPCR; GPCR, family 1. Chromosomal Location of Human Ortholog: 12p13.31. Cellular Component: cytosol; integral to plasma membrane; plasma membrane. Molecular Function: C3a anaphylatoxin receptor activity; complement component C3a binding; complement component C3a receptor activity; G-protein coupled receptor activity; phosphoinositide phospholipase C activity. Biological Process: blood circulation; chemotaxis; complement receptor mediated signaling pathway; elevation of cytosolic calcium ion concentration; G-protein coupled receptor protein signaling pathway; inflammatory response; positive regulation of angiogenesis; positive regulation of smooth muscle cell proliferation; regulation of blood pressure; tolerance induction to nonself antigen

size1 :

48-Strip-Wells

price1 :

300 USD

size2 :

96-Strip-Wells

price2 :

415

size3 :

5x96-Strip-Wells

price3 :

1825

size4 :

10x96-Strip-Wells

price4 :

3425