ELISA/assay

Human Resistin ELISA Kit

MBS762836

48-Strip-Wells

300 USD

ELISA Kit

Human Resistin ELISA Kit

[Resistin]

[Resistin/RETN/ADSF/Adipose tissue-specific secretory factor/HXCP1/RETN1/RSTNXCP1]

[resistin; Resistin; resistin; resistin; Adipose tissue-specific secretory factor; ADSF; C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein; Cysteine-rich secreted protein A12-alpha-like 2; Cysteine-rich secreted protein FIZZ3]

[RETN]

[RETN; RETN; ADSF; RSTN; XCP1; FIZZ3; RETN1; FIZZ3; HXCP1; RSTN; ADSF]

RETN_HUMAN

Human

108

This assay has high sensitivity and excellent specificity for detection of RETN. No significant cross-reactivity or interference between RETN and analogues was observed.

Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich ELISA, Double Antibody. Detection Range: 31.2-2000pg/ml. Sensitivity: 18.75pg/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level RETN were tested 20 times on one plate, respectively. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level RETN were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- RETN antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- RETN antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the RETN amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of RETN can be calculated.

12584202

AAG59824.1

8,686 Da

Adipogenesis Pathway (198832)

This gene belongs to the family defined by the mouse resistin-like genes. The characteristic feature of this family is the C-terminal stretch of 10 cys residues with identical spacing. The mouse homolog of this protein is secreted by adipocytes, and may be the hormone potentially linking obesity to type II diabetes. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2010]

resistin: Hormone that seems to suppress insulin ability to stimulate glucose uptake into adipose cells. Potentially links obesity to diabetes. Belongs to the resistin/FIZZ family. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 19p13.2. Cellular Component: extracellular space; nucleus. Molecular Function: hormone activity. Biological Process: aging; fat cell differentiation; positive regulation of smooth muscle cell migration; positive regulation of smooth muscle cell proliferation; positive regulation of synaptic transmission; response to insulin stimulus; response to mechanical stimulus. Disease: Diabetes Mellitus, Noninsulin-dependent

48-Strip-Wells

300 USD

96-Strip-Wells

415

5x96-Strip-Wells

1825

10x96-Strip-Wells

3425