catalog number :
MBS762829
products type :
ELISA Kit
products full name :
Porcine N-Terminal Pro-Brain Natriuretic Peptide ELISA Kit
products short name :
[N-Terminal Pro-Brain Natriuretic Peptide]
products gene name :
[NT-ProBNP]
specificity :
This assay has high sensitivity and excellent specificity for detection of Porcine NT-ProBNP. No significant cross-reactivity or interference between Porcine NT-ProBNP and analogues was observed.
storage stability :
Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).
image1 heading :
Typical Testing Data
image2 heading :
Typical Standard Curve
other info1 :
Samples: Serum, plasma and other biological fluids. Assay Type: Sandwich ELISA, Double Antibody. Detection Range: 78.125-5000pg/ml. Sensitivity: 46.875pg/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Porcine NT-ProBNP were tested 20 times on one plate, respectively. Intra-Assay: CV<8%. Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Porcine NT-ProBNP were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<10%
products description :
Principle of the Assay This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Porcine NTProBNP antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Porcine NTProBNP antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Porcine NT-ProBNP amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Porcine NT-ProBNP can be calculated.
size4 :
10x96-Strip-Wells